关键词: PinX1, 原发性肝细胞肝癌, 预后, 实时荧光定量PCR

Abstract: 【Abstract】〓Objective〓To explore the mRNA expression of Pin2/TRF1-interacting protein X1(PinX1) in hepatocellular carcinoma tissues as well as its prognostic significance. Methods〓A total of 43 paired hepatocellular carcinoma specimens and their adjacent normal tissues with corresponding patients’.clinical data were collected..The mRNA expression of PinX1 was detected by real-time quantitative polymerase chain reaction.(Q-PCR),.and its correlations with pathological features was evaluated by chi-square test,.prognosis were further analyzed by Kaplan-Meier method..Cox proportional hazard model was used to estimate relative risks resulting in a poor prognosis. Results〓In the whole specimens,.PinX1 mRNA expression was decreased in 29 tumor tissues.(67.4%),.while only 14 adjacent normal tissues were in low expression with a statistically significance(P<0.05); there was a correlation between PinX1 mRNA expression and patient’s age; low mRNA expression of PinX1 in tumor tissues were crucially associated with patients’.overall survival by univariate and multivariate Cox proportional-hazards regression analysis.(HR=5.057,P=0.005;.HR=3.761,P=0.006),.as the same results as in survival analysis..Conclusion〓PinX1 is a potential tumor suppressor gene that inhibits the onset and progression of HCC,.with a prospect that it may be an important biomarker in surveillance of prognosis and treatment.

Key words: PinX1, Hepatocellular carcinoma, Prognosis, Real-time quantitative polymerase chain reaction