关键词: 颅脑损伤, 脑疝, CD8+CD28-T细胞, 细胞因子

Abstract: 【Abstract】〓Objective〓To investigate the dynamic changes of peripheral blood CD8+CD28-T cells and clinical significance in patients with cerebral hernia. Methods〓A total of 77 patients of severe brain injury were divided into two groups: cerebral hernia (A group, n=36) and no-cerebral hernia (B group, n=41), 32 healthy subjects were in control group (C group), the level of CD4+ CD25+ and the percentage of TGF-β1, IL-10 in peripheral blood was analyzed with flow cytometry. All variables were observed in group A and B at post-traumatic 3, 6, 12 h. Results〓Difference of three groups in CD8+ CD28- T cells, TGF-β1 and IL-10 overall was significant (P<0.05), but no statistically significant difference between group B and group C, all variables in group A was significantly lower than group B and group C (P<0.05). The level of CD8+ CD28-T cells, TGF-β1 and IL-10 had no significant difference in group B for 3, 6, 12 h. Compared with group B, CD8+ CD28- T cells, TGF-β1 and IL-10 in group A of three different time points showed a progressive decline, difference between any two time points was significant. Conclusion〓One important cellular immunological characteristics of cerebral hernia is the significant decrease of CD8+ CD28-T cells, especially the progressive decline of which within 12 h are closely related to disease progression.

Key words: Craniocerebral injury, Cerebral hernia, CD8+ CD28- T cells, Cytokines