关键词: 重症急性胰腺炎, 大鼠, 凝血功能, 血钙离子, TNF-α抑制剂

Abstract: 【Abstract】〓Objective〓To investigate the effect of TNF-α inhibitor on coagulation function in a model of rats with severe acute pancreatitis (SAP). Methods〓SAP rat model was made. The control group rats (sham-injected group, n=20) were prepared to be made into SAP model with a small amount of saline injection. Sixty rats for SAP model: 20 rats for therapy group 1, group 2, group 3 respectively. After the model was successfully made, TNF-α inhibitor was injected into SAP model through dorsal vena of penis by dose levels of 0.15 mg/kg, 0.30 mg/kg and 0.45 mg/kg respectively.After 24 hrs, anesthesia was performed, and 3 ml common carotid artery blood of rats in all groups were extracted for the tests of coagulation and calcium related. Results〓During the test, all rats survived. Rats in therapy group were in better status, some of them still had autonomic activities and feeding. APPT and FIB level in therapy group 1, group 2 and group 3 were lower than that of sham-injected group, and comparative differences of PT, APPT, FIB, serum calcium among three therapy groups have statistical significance (P<0.05). Between the 3 groups of serum total calcium difference within treatment groups was not statistically significant (P>0.05). Conclusion〓SAP can cause declined coagulation function of rats and serum calcium level decreased. TNF-α inhibitor helps to improve the rats' coagulation function of SAP model, and the mechanism of action is related to fluid infusion and inhibiting inflammatory reaction and a quite significant dose-dependent is represented.

Key words: Severe acute pancreatitis, Rats, Coagulation function, Calcium, TNF-α inhibitor