关键词: 组蛋白, 胆道梗阻, 肝功能

Abstract: 【Abstract】 Objective〓To study the effect of histone preconditinoning on liver function in biliary obstruction rats. Methods〓104 Wistar rats were randomized into 4 group, normal group (group A), sham operation group (group B), bile duct ligation group (group C) and the His group (group D, (200 μg/kg of histone proteins was administered intraperitoneally 24 hr before subjected to BDL). After the surgery, blood samples and live tissue specimens were collected at the time points of 0 h, 12 h, 24 h, 48 h and 72 h. Serum ALT, TB and DB levels were detected. Histological changes of liver tissues were analyzed. Serum IL-6, TNF-α and His levels were detected by ELISA. The mRNA expression levels of TLR-4 in liver Kupffer cells was examined by reverse transcription polymerase chain reaction (RT-PCR). Results〓The pathological scores in C group were higher than those of A and B group (P<0.01) and D group (P<0.05). The serum IL-6 and TNF-α levels in C group were higher than those of B group and group D at each time (P<0.01, P<0.05). Compared to B group, the TLR4 mRNA expression in Kupffer Cells were increased in C group with significant difference (P<0.01). In D group, the mRNA expression levels of TLR4 in Kupffer Cells were lower than those of C group at each time point (P<0.05). Conclusion〓In our study, histone preconditioning protected against liver injury in biliary obstruction rats. The levels of IL-6 and TNF-α were suppressed by activate the TLR4 pathway in Kupffer cells, local and systemic inflammatory response were suppressed.

Key words: Histone, Biliary obstruction, Liver function