关键词: 微小RNA126, 肝癌, 细胞增殖, 细胞凋亡, PIK3R2

Abstract: 【Abstract】〓Objective〓To explore the influence of microRNA-126 (miR-126) on proliferation and apoptosisof human hepatocellular carcinoma cell line SMMC-7721 and evaluate the correlation between miR-126 and PIK3R2. Methods〓After transfection of microRNA mimics into SMMC-7721 cells,cell proliferation and apoptosis were assessed by CCK-8 assay and cell apoptosis assay. Expression of PIK3R2 was evaluated by quantitative real-time polymerase chain reaction and western blotting. Luciferase reporter assay was used to confirm the relationship between miR-126 and PIK3R2. Results〓The outcomes by CCK-8 assay and apoptosis assay revealed that transfection of miR-126 suppressed cell proliferation, induced cell apoptosis of SMMC-7721 cells. Ectopic overexpression of miR-126 caused significant decrease of protein level of PIK3R2 (P=0.0135). In addition, luciferase assay showed that miR-126 significantly weakened the normalized PIK3R2-3’ UTR luciferase activity (P=0.0016). Conclusion〓Overexpressed miR-126 can inhibit proliferation and promote apoptosis of hepatocellular carcinoma cells by downregulating PIK3R2, thus indicating its tumor suppressive function in hepatocellular carcinoma.

Key words: microRNA-126, Hepatocellular carcinoma, Cell proliferation, Cell apoptosis, PIK3R2.