关键词: 肾癌, CD8+CD28-T细胞, 分期, 流式细胞术

Abstract: 【Abstract】〓Objective〓To investigate the expression and clinical significance of CD8+CD28-T cells for patients with renal carcinoma undergoing nephrectomy. Methods〓Fifty-two patients who underwent radical prostatectomy were collected as research objects (group A). Flow cytometry was used to test peripheral blood CD8+CD28-T cells before and after operation, interleukin 10 (IL-10), and transforming growth factor beta 1 (TGF-beta 1) content. The correlation between the three levels and of tumor stage was analyzed. The patients with renal hamartoma (group B) and normal people (group C) served as controls. Results〓The CD8+CD28-T cells number, expression of IL-10 and TGF-beta 1 in group A were significantly higher than the other two groups, but had no statistical difference between the group B and group C. Preoperative levels of CD8+CD28-T cells, IL-10 and TGF-beta 1 in the patients with stage III were significantly higher than those with stage I and stage II(P<0.05). Postoperative levels of CD8+CD28-T cells,IL-10 were increased and TGF-beta 1 content was decreased in patients with stage III than that of preoperative period (P<0.05), but no difference in patients with stage I and stage II between pre- and post-operation. Correlation analysis showed that the CD8+CD28-T cells, IL-10 and TGF-beta 1 and tumor stage showed significant positive correlation (P<0.05) and the related degree was CD8+CD28-T cells > TGF-beta 1> IL-10. Conclusion〓The increases of CD8+CD28-T cells and cytokines levels could be a new immune mechanism of kidney carcinoma, and the former could identify kidney cancer and benign tumor, evaluate the operation effect.

Key words: Renal carcinoma, CD8+CD28-T cells, Tumor Stage, Flow cytometry