关键词: 血栓闭塞性脉管炎, 动脉硬化性闭塞症, 调节性T细胞, 抗中性粒细胞, 细胞因子

Abstract: 【Abstract】〓 Objective〓To investigate the expression and significance of CD4+CD25+ regulatory T cells for the patients with thromboangiitis obliterans (TAO). Methods〓Blood samples were collected from 32 patients with TAO, 35 patients with arteriosclerosis obliterans (ASO) (positive control) and 35 healthy people(normal control)..Treg,.transforming growth factor beta 1(TGF-beta 1)and interleukin 10(IL-10)..were detected by flow cytometry. Cytoplasmic antineutrophil cytoplasmic antibody (CANCA) and perinuclear anti neutrophil cytoplasmic antibody (PANCA) were determined by ELISA method. Results〓Compared with normal control group, Treg and TGF-beta 1 in TAO group were significantly increased(P<0.05),while those were significantly lower in ASO patients than that of normal control group. The level of IL-10 in TAO and ASO patients were lower than that in normal controls and there were no statistical difference between TAO and ASO patients. The expression of both CANCA and PANCA in TAO patients were increased significantly than that of normal control group, but there was no statistical difference between ASO patients and normal volunteers. Pearson correlation analysis showed that Treg and TGF-beta 1 and IL-10 either one with CANCA and PANCA had significant positive correlation (P<0.05). Conclusion〓Treg and TGF-beta 1 may be involved in the pathogenesis of TAO.

Key words: Thromboangiitis obliterans, Arteriosclerosis obliterans, Regulatory T cells, Anti neutrophil, cytokines