关键词: 热二氧化碳, 树突状细胞, 胃癌, 增殖抑制

Abstract:

【Abstract】〓Objective〓To explore the role of dendritic cells (DC)-derived dexosome (DEX) in proliferation regulation of human gastric adenocarcinoma cells after treated with hyperthermic CO₂. Methods〓Based on an in vitro hyperthermic CO₂ pneumoperitoneum experimental model,the DC-derived DEX was isolated and identified through TEM after treatment by hyperthermic CO₂. AGS cells were co-cultured with DEX in vitro. And the cell proliferation was detected by CCK-8 assay,the expression of p-AKT and bcl-2 in AGS cells was examined by Western blot, and relative activity of p-AKT and bcl-2 was determined by colorimetric assay. Results〓DEX was successfully isolated. The proliferation rate of AGS cells was 54.73%±2.04% in hyperthermic CO₂ treated group, lower than that of heat treated group and control group (P<0.05). Significantly lower expression of p-AKT and bcl-2 in AGS cells treated with hyperthermic CO₂-DEX was observed by Western blot. And the relative activity of p-AKT and bcl-2 in AGS cells treated with hyperthermic CO₂-DEX were 41.28%±2.31% and 36.49%±1.92%. Conclusions〓DC-derived DEX treated by hyperthermic CO₂ could significantly inhibit proliferation of AGS cells, which may be related to the inhibition of AKT pathway activation and the decrease of expression of bcl-2.

Key words: Hyperthermic CO2, Dendritic cell, Gastric cancer, Proliferation inhibition