CGRP在糖尿病足神经与骨膜中的表达及其对破骨细胞分化及金属蛋白酶表达的影响

doi:10.3969/j.issn.1009-976X.2014.02.005

岭南现代临床外科 ›› 2014, Vol. 14 ›› Issue (02): 132-135.DOI: 10.3969/j.issn.1009-976X.2014.02.005

CGRP在糖尿病足神经与骨膜中的表达及其对破骨细胞分化及金属蛋白酶表达的影响

刘文宙¹,陈炳豪²,Aditya Anand¹,程翔宇²,杨涛¹,宋卫东²

1. 中山大学孙逸仙纪念医院
2. 孙逸仙纪念医院

通讯作者: 刘文宙
基金资助:
骨髓基质干细移植后糖尿病神经病变大鼠RANK/RANKL/OPG轴与骨愈合相关性的实验研究

The expression of CGRP in nerve fiber and periosteum of diabetic foot and its effects on osteoclasts differentiation and MMPs expression

Liu Wenzhou, Chen Binghao, Aditya Anand, Chen Xiangyu, Yang Tao, Song Weidong

Received:2014-02-19 Revised:2014-03-27 Online:2014-04-20 Published:2014-04-20

摘要/Abstract

摘要： 【摘要】〓目的〓研究降钙素基因相关肽(CGRP)在糖尿病足神经与骨膜中的表达及其对破骨细胞分化及金属蛋白酶(MMPs)表达的影响。方法〓取糖尿病足截肢病人与车祸导致截肢病人组织作CGRP免疫组化,以了解其在糖尿病足周围神经与跟骨骨膜中的表达。用CGRP处理RAW264.7培养体系,了解其对破骨细胞分化及金属蛋白酶表达的影响。结果〓糖尿病足周围神经纤维与骨膜组织中CGRP表达下降,CGRP可抑制RAW264.7细胞向破骨细胞分化及抑制,MMP-3,MMP-9的表达。结论〓糖尿病周围神经病变导致的CGRP水平的下降,可使破骨细胞的活化与MMPs的表达失去调节,与异常的骨破坏和难治性溃疡有着密切的关系,在糖尿病足的发病机制中起着重要作用。

关键词: 糖尿病足, CGRP, 破骨细胞, 金属蛋白酶

Abstract: 【Abstract】〓Objective〓To detect the expression of calcitonin gene-related peptide (CGRP) in nerve fiber and periosteum of diabetic foot and its effects on osteoclasts differentiation and Metal Matrix Proteinases (MMPs) expression. Methods〓Immunohistochemical staining was utilized to detect the expression of CGRP in nerve fibers and periosteum of diabetic foot patients and traumatic amputees (as controls). RAW 264.7 cells were treated with CGRP to study its effects on osteoclasts differentiation and MMPs expression.TRAP staining was used to determine osteoclasts and western-blot was utilized to detect the expression of MMP-2,MMP-3 and MMP-9. Results〓The expression of CGRP in nerve fibers and periosteum was deceased in Diabetic foot patients.CGRP inhibited the differentiation of osteoclasts and decreases the expression of MMP-3 and MMP-9. Conclusion〓The decreased CGRP in diabetic foot can result to MMPs over-expression of osteoclasts due to activation,leading to bone destruction and refractory foot ulceration and may play an important role in the pathogenesis of diabetic foot.

Key words: Diabetic foot, CGRP, Osteoclasts, MMPs

中图分类号:

R681.8

刘文宙陈炳豪 Aditya Anand 程翔宇杨涛宋卫东. CGRP在糖尿病足神经与骨膜中的表达及其对破骨细胞分化及金属蛋白酶表达的影响[J]. 岭南现代临床外科, 2014, 14(02): 132-135.

Liu Wenzhou, Chen Binghao, Aditya Anand, Chen Xiangyu, Yang Tao, Song Weidong. The expression of CGRP in nerve fiber and periosteum of diabetic foot and its effects on osteoclasts differentiation and MMPs expression[J]. Lingnan Modern Clinics in Surgery, 2014, 14(02): 132-135.

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CGRP在糖尿病足神经与骨膜中的表达及其对破骨细胞分化及金属蛋白酶表达的影响

The expression of CGRP in nerve fiber and periosteum of diabetic foot and its effects on osteoclasts differentiation and MMPs expression

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