关键词: 膀胱肿瘤, MALAT1, RNA干扰, 基因芯片, 癌症通路

Abstract: 【Abstract】〓Objective〓To investigatethe effect of MALAT1 on regulationof proliferation, migration, invasion, clone formation, and other functions in UMUC3 cell lines of bladder cancer, and to explore the molecular mechanism of MALAT1 in bladder cancer. Methods〓Thesmall interfering RNA (siRNA) was designed and synthesized and then transfected into the UMUC3 cells. The transfection efficiency was detected with RT-QPCR assay. MTS assay, transwell migration and matrigel invasion assay, colony formation assay and 5-ethinyl-2′-deoxyuridine (EDU) assay were performed to evaluate the proliferation, migration, invasion, clone formation and cell cycle respectively. To explore the potential pathways of MALAT1, we performed gene chip assay. Results〓After down regulation the expression of MALAT1, the proliferation, invasion, migration and colony-forming ability of UMUC3 bladder cancer cells were impaired. Gene chip found that cancer pathways related gene changed obviously after knockdown of MALAT1 in UMUC3 cells. Conclusion〓MALAT1 promoted the proliferation, invasion, migration, cell cycle and clone formation ability in UMUC3 cell lines of bladder cancer through modulating the cancer pathways related gene activation and deactivation.

Key words: Bladder cancer, MALAT1, RNA interference, Gene chip, Pathway in cancer