白细胞抗原G在结肠癌中的表达及意义

doi:j.issn.1009-976X.2013.02.007

摘要/Abstract

摘要：

【摘要】目的研究结肠癌切除标本中远癌组织、癌旁组织及癌组织白细胞抗原G(HLA-G)蛋白表达情况,探讨HLA-G在结肠癌发生、发展中的作用。方法应用免疫组化SP法,检测HLA-G 蛋白在结肠癌远癌组织、癌旁组织及癌组织的表达情况。结果癌组织与癌旁组织、远癌组织比较具有统计学意义(P<0.01),癌旁组织与远癌组织比较具有统计学意义(P<0.01)。 HLA-G 与结肠癌血管侵犯、淋巴结转移及临床病理分期有关(P<0.01),而与性别、年龄、肿块大小、肿块部位、癌胚抗原(CEA)水平及组织学分级无关。结论 HLA-G 在结肠癌中高表达。HLA-G 在血管侵犯组、淋巴结转移组及临床分期较晚组表达显著升高,这些因素可能与结肠癌的侵袭和转移有关。

关键词: 结肠癌, 人白细胞抗原G, 免疫组化, 癌胚抗原

Abstract:

【Abstract】 Objective To study the expression of -G protein in normal tissues, normal paracarcinoma tissues and carcinoma tissues derived from colon carcinoma specimens and to explore its effects with occurrence and development of colon carcinoma. Methods Immunohistochemical method was used to detect the expression of -G protein in distal tissues, paracarcinoma tissues and carcinoma tissues of colon carcinoma. Results There were significant differences of the expression of -G protein in carcinoma tissues compared with distal tissues or paracarcinoma tissues (P<0.01) and significant difference between paracarcinoma tissues and distal tissues (P<0.01). The expression of -G protein in colon carcinoma tissue was positively correlated with tumor vascular invasion, lymph node metastasis and clinical staging (P<0.01), without relationship gender, age, tumor size, tumor location, histological grade, and preoperative CEA. Conclusion -G expression was progressively increased from distal tissue,paracarcinomatissue to colon carcinoma tissues,which associates with the tumor vascular invasion, lymph node metastasis, clinical staging.

Key words: Colon carcinoma, HLA-G, Immunohistochemistry, CEA

中图分类号:

R735.3⁺5

张广献杨海峰曾宪成*. 白细胞抗原G在结肠癌中的表达及意义[J]. 岭南现代临床外科, 2013, 13(02): 99-102.

Zhang Guangxian; Yang Haifeng; Zeng Xiancheng. Expression and its significance of HLA-G in colon carcinoma[J]. Lingnan Modern Clinics in Surgery, 2013, 13(02): 99-102.

参考文献

[1] LE BOUTEILLER P. HLA-G in the human placenta: expression and potential functions [J]. Biochem Soc Trans, 2000, 28(2): 208-212.
[2] CHANG CC, CAMPOLI M, FERRONE S. HLA class Ⅰantigen expression in malignant cells:why does it not always correlate with CTL-mediated lysis [J]. Curr Opin Immunol, 2004, 16(5): 644-650.
[3] ROUAS-FREISS N, MOREAU P, FERRONE S, et al. HLA-G proteins in cancer: do they provide tumor cells with an escape mechanism [J]. Cancer Res, 2005, 65(22): 10139-10144.
[4] CAO M, YIE SM, LIU J, et al. Plasma soluble HLA-G is a potential biomarker for diagnosis of colorectal, gastric, esophageal and lung cancer [J]. Tissue Antigens, 2011, 78(2):120-128.
[5] YAN W, ZHOU Y, MENG XQ, et al. Expression of HLA-G in patients with hepatocellular carcinoma [J]. Hepatobiliary Pancreat Dis Int, 2011, 10(2):158-163.
[6] SCHüTT P, SCHüTT B, SWITALA M, et al. Prognostic relevance of soluble human leukocyte antigen-G and total human leukocyte antigen class I molecules in lung cancer patients [J]. Hum Immunol, 2010, 71(5):489-495.
[7] MACH P, BLECHARZ P, BASTA P, et al. Differences in the soluble HLA-G blood serum concentration levels in patients with ovarian cancer and ovarian and deep endometriosis [J]. Am J Reprod Immunol, 2010, 63(5):387-395.
[8] ESTHER M, ANITA S, JOHANNA G, et al. HLA-E and HLA-G expression in classical HLA ClassI-Negative tumors is of prognostic value for clinical outcome of early breast cancer patients [J]. J Immunol, 2010, 185(12):7452-7459.
[9] JUNG YW, KIM YT, KIM SW, et al. Correlation of human leukocyte Antigen-G (HLA-G) expression and disease progression in epithelial ovarian cancer [J]. Reproductive Sciences, 2009, 16(11):1103-1111.
[10] CAROSELLA ED, FAVIER B, ROUAS-FREISS N, et al. Beyond the increasing complexity of the immunomodulatory HLA-G molecule[J]. Blood, 2008, 111(10):4862-4870.
[11] YANRONG YU, YUN WANG, MEIFU FENG, et al. Human leukocyte antigen–G1 inhibits natural killer cytotoxicity through blocking the activating signal transduction pathway and formation of activating immunologic synapse[J]. Human Immunol, 2008, 69(1):16-23
[12] CONTINI P, GHIO M, MERLO A, et al. Apoptosis of antigen-specific T lymphocytes upon the engagement of CD8 by soluble HLA class I molecules is Fas ligand/Fas mediated: Evi-dence for the involvement of p56lck, calcium calmodulin kinaseII, and calcium-independent protein kinase C signaling pathways and for NF-kappa Band NF-AT nuclear translocation[J]. J Immunol, 2005, 175(11):7244-7254.
[13] 曾宪成, 张彤, 李华, 等. RNA干扰白细胞抗原-G增强自然杀伤细胞对肝癌的免疫监视[J]. 中华实验外科杂志, 2009, 26(8):991-994.

[1]	赵昭, 阎晓玲. ESM-1在复发性垂体腺瘤中的表达及意义[J]. 岭南现代临床外科, 2022, 22(1): 94-97.
[2]	胡明超, 朱宝松. NDC1基因在结肠癌中诊断及预后价值的生物信息学分析：一项基于TCGA数据库的研究[J]. 岭南现代临床外科, 2021, 21(06): 598-602.
[3]	邹军, 杨淑婷, 王侃, 赵子然. 基于倾向性评分匹配方法的结肠癌根治术后发生吻合口漏的围术期相关危险因素分析[J]. 岭南现代临床外科, 2021, 21(05): 551-555.
[4]	方凯, 伍衡, 雍娟娟, 张建龙, 曾育杰. 1例乙状结肠癌伴肝转移患者的多学科诊疗[J]. 岭南现代临床外科, 2021, 21(02): 151-156.
[5]	庄志浩, 欧阳秋伟, 陶世明, 霍景山, 吴岷翰, 韩新峰, 贺友. 老年患者应用自膨式金属支架作为结肠癌急性恶性肠梗阻择期手术的临时处理手段的短期评价[J]. 岭南现代临床外科, 2020, 20(04): 421-424.
[6]	张平, 彭晓锋, 林建涛, 王建锐, 陈金欣, 林峻岭. 腹腔镜技术在梗阻性左半结肠癌中的应用体会[J]. 岭南现代临床外科, 2020, 20(04): 435-439.
[7]	高晓琳, 修霞, 邱萍萍, 张杜合一, 谢舒乐, 林钊宇. FOS样抗原1在头颈鳞癌中的表达及临床意义[J]. 岭南现代临床外科, 2020, 20(03): 319-323.
[8]	刘宇峰, 刘少杰, 刘丹. 手辅助腹腔镜左半结肠癌根治术的临床实践（附81例报告）[J]. 岭南现代临床外科, 2020, 20(02): 172-175.
[9]	陈景森，欧希，赵洋，余光银，刘晓平*. LAP+CD4+T细胞在肝癌微环境中的分布特点及意义[J]. 岭南现代临床外科, 2019, 19(05): 528-532.
[10]	谭明华，胡海涛，区小卫，廖建南，冯伟清，朱劲涛. ERAS理念对老年腹腔镜结肠癌术后高敏-C反应蛋白和前白蛋白的影响[J]. 岭南现代临床外科, 2019, 19(05): 552-554.
[11]	梁家宏，许侨东，严江*. 结直肠癌中FAM135B 基因的表达及功能的生物信息学分析[J]. 岭南现代临床外科, 2018, 18(03): 265-268.
[12]	施景龙林显敢蓝球生许鹤洋. 肿瘤抑制基因 NDRG2 调节结肠癌细胞糖酵解抑制结肠癌细胞增殖的研究[J]. 岭南现代临床外科, 2017, 17(04): 407-409.
[13]	曹杰智赖东明伍衡褚忠华曾育杰. 在结肠癌细胞中P53突变调控 KLF5功能及表达变化的实验研究[J]. 岭南现代临床外科, 2017, 17(04): 410-414.
[14]	王道笃何永越万德炎石一峰. 左半结肠癌合并急性肠梗阻Ⅰ期吻合手术治疗体会[J]. 岭南现代临床外科, 2017, 17(04): 420-422.
[15]	施景龙罗兴喜许鹤洋蓝球生黄永亮褚忠华 . 肝再生磷酸酶-3上调转化生长因子-beta促进结肠癌LoVo细胞侵袭的机制研究[J]. 岭南现代临床外科, 2017, 17(03): 287-294.