关键词: 蛋白二硫键异构酶家族成员4, 胶质瘤, 凋亡

Abstract: Objective To investigate the expression of protein disulfide isomerase family A member 4（PDIA4） in glioma and the effect on apoptosis. Methods The RNAseq data was downloaded from CGGA database. Glioblastoma cell lines（U251,U87） and human normal astrocyte cell line HA1800 were used in the study. The relative expression of PDIA4 in glioma cell lines was detected by RT-qPCR. U251 cells were transfected with siRNA, transfected with siPDIA4 as si#768 group and si#1829 group, untransfected as negative control group, and transfected with siNC as blank control group. Then alarma blue assay and colony formation assay were used to examine the proliferation of U251 cells, the apoptosis rate was detected by flow cytometry, and Western blotting was used to detect the expression of cleaved-caspase3、cleaved-caspase7 protein. Results In CGGA database, the expression level of PDIA4 were different in gliomas of different grades （P<0.05）, the five-year survival rates of the high PDIA4 expression group was lower than the low expression group, and Log-rank test（Log-rank=264.32, P<0.001）. Compared with normal astrocyte cell line HA1800, the relative expression levels of PDIA4 was increased in U251 and U87（F=58.44, P<0.05）. Compared with blank group and negative control group, proliferation and colony formation ability of si#768 group and si#1829 group were inhibited（P<0.01）, and apoptosis rates （17.67±0.11%, 19.50±2.30% vs. 3.72±2.11%, 6.53±0.13%, F=51.06, P<0.01） were significantly increased. Compared with the negative control group, the protein expression of cleaved-caspase-7 were increased up to 218.53%and 169.68% （F=12.61, P<0.05）. Conclusion Knockdown of PDIA4 may promotes the apoptosis of glioma cells by activating caspase pathway.

Key words: PDIA4, glioma, apoptosis