关键词: 8-硝基鸟嘌呤, 肝内胆管细胞癌, 肝吸虫感染, 细胞凋亡

Abstract: Objective To study expression and significance of 8-Nitroguanine in intrahepatic cholangiocarcinoma with liver fluke infection. Methods Expression of 8-nitroguanine in 18 intrahepatic cholangiocarcinoma tissue was detected by enzyme-linked immunosorbent assay （ELISA）, including paracancerous tissue and normal liver tissue. Serum levels of 8-nitroguanine in intrahepatic cholangiocarcinoma patients preoperatively and 18 clonochiasis sinensis infected patients and 18 healthy volunteers were measured by ELISA. RBE cells were treated with different concentrations of 8-nitroguanine and the 50% inhibiting concentration （IC₅₀） was determined by MTT assay. Invasive ability of RBE cell treated with 8-nitroguanine was detected by Transwell assay. Results In 18 intrahepatic cholangiocarcinoma, 8-Nitroguanine levels were significantly higher in tumor tissues than in peritumoral tissues and in normal liver tissues （P<0.05）, and there was no difference between in paracancerous tissues and in normal liver tissues（P>0.05）. And 8-Nitroguanine levels in serum, there was no difference comparable between in intrahepatic cholangiocarcinoma patients preoperatively and clonochiasis sinensis infected patients and 18 healthy volunteers（P>0.05）. 8-Nitroguanine suppressed the proliferation of RBE cells in a dose-dependent manner, but has no effect on cell invasion （P>0.05）. Conclusions Overexpression of 8-Nitroguanine in intrahepatic cholangiocarcinoma with liver fluke infection may be involved in the oncogenesis of disease, instead of tumor progression.

Key words: 8-Nitroguanine, intrahepatic cholangiocarcinoma, liver fluke infection, apoptosis