关键词: 细胞周期, 细胞休眠期, 染色质行为, 分裂间期

Abstract: Objective Dysregulation of the cell cycle can lead to genome instability, causing malignant transformation and canceration of cells. By analyzing the dynamic behavior characteristics of chromatin from G0 phase to the interphase of the breast cancercell linesinour study, it provides a certain experimental basis for further research on cancer cell cycle regulation. Methods In mouse breast cancer 4T1 cellline, we applied DNA markers and histone-related fluorescent markers to reflect the chromatin morphology in the cell and its dynamic changes in the cell cycle, recording and analyzing experimental graphicsby laser confocal microscope. Results Our study showed that the DAPI signal and GFP-H2B signal have better co-localization, and both can effectively label chromatin. During the period from the G0 phase to the interphase, a large volume of fluorescently labeled plaques was observed at the beginning, in which there were multiplecentromere satellite DNA in situ hybridization spots. The fluorescent plaques gradually dissociate into small plaques even disappeared but reappearedwith a smaller volume 1 h 40 mins later, whichwas regularly arranged on the periphery of the nucleolus and the inside of the nuclear membrane. Conclusion There is aggregation between chromatin in G0 phase breast cancer cells, and it may be related to the function of centromeric satellite DNA. When entering the interphase under the stimulation of cell proliferation signal, the aggregate will gradually dissociate and disappear, after the chromatin is copied and highly compressed to form a chromosome, it further divides into two cells.

Key words: interphase, cell cycle, chromatin behavior, G0 phase