POLE2对非小细胞肺癌细胞侵袭迁移能力的影响

doi:10.3969/j.issn.1009-976X.2020.03.009

岭南现代临床外科 ›› 2020, Vol. 20 ›› Issue (03): 307-310.DOI: 10.3969/j.issn.1009-976X.2020.03.009

POLE2对非小细胞肺癌细胞侵袭迁移能力的影响

姬路鹏¹, 彭安萍², 张毅^3,*, 梁庭都¹

1.珠海市第五人民医院内科,广东珠海519055;
2.中山大学孙逸仙纪念医院南校园门诊部,广东广州510120;
3.珠海市人民医院呼吸科,广东珠海519000

通讯作者: *张毅,Email: 231917657@qq.com
基金资助:
珠海市卫计局医学科研基金（2016-25）

Effect of overexpression of POLE2 on invasion and migration of non-small cell lung cancer cells

JI Lu-peng¹, PENG An-ping², ZHANG Yi³, LIANG Ting-du¹

1. Department of Medicine, The Fifth People's Hospital of Zhuhai, Guangdong 519055, China;
2. South Campus Clinic, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China;
3. Department of Respiratory Medicine, Zhuhai People's Hospital, Zhuhai, Guangdong 519000, China

Received:2020-03-11 Online:2020-06-20 Published:2020-06-20
Contact: ZHANG Yi, 231917657@qq.com

摘要/Abstract

摘要： 目的探讨POLE2对非小细胞肺癌（NSCLC）细胞侵袭迁移能力的影响。方法采用实时荧光定量PCR（qRT-PCR）检测人支气管上皮样细胞BEAS-2B和NSCLC细胞A549、SPC-A1中POLE2的表达水平。构建POLE2过表达细胞模型,qRT-PCR和蛋白质印迹法（Western blot）验证转染效率。采用Cell Counting Kit-8（CCK-8）实验检测NSCLC细胞的增殖能力,Transwell实验检测NSCLC细胞的侵袭迁移能力,qRT-PCR检测MMP9的表达水平。结果人NSCLC细胞SPC-A1、A549中POLE2的表达水平显著高于人支气管上皮样细胞BEAS-2B（P<0.05）。过表达POLE2促进了A549细胞的增殖和侵袭迁移,上调了MMP9的表达（P<0.05）。结论过表达POLE2可能通过上调MMP9促进NSCLC细胞的侵袭迁移。

关键词: 迁移, 侵袭, NSCLC, POLE2

Abstract: Objective To investigate the effect of POLE2 on the invasion and migration of non-small cell lung cancer （NSCLC） cells. Methods The expression of POLE2 in human NSCLC cells BEAS-2B and A549,human bronchial epithelial cell line SPC-A1 was detected by real-time PCR （qRT-PCR）. The POLE2-overexpression cell was established. The transfection efficiency was verified by qRT PCR and Western blot. Cell counting kit-8 （CCK-8） was used to detect the proliferation of NSCLC cells, Transwell was used to detect the invasion and migration of NSCLC cells, and qRT-PCR was used to detect the expression of MMP9. Results The expression level of POLE2 in human NSCLC cells SPC-A1 and A549 was significantly higher than that of BEAS-2B （P<0.05）. Overexpression of POLE2 promoted the proliferation, invasion and migration of A549 cells, and upregulated the expression of MMP9（P<0.05）. Conclusion Overexpression of POLE2 may promote the invasion and migration of NSCLC cells by up regulating MMP9.

Key words: migration, POLE2, invasion, non-small cell lung cancer

中图分类号:

R734.2

姬路鹏, 彭安萍, 张毅, 梁庭都. POLE2对非小细胞肺癌细胞侵袭迁移能力的影响[J]. 岭南现代临床外科, 2020, 20(03): 307-310.

JI Lu-peng, PENG An-ping, ZHANG Yi, LIANG Ting-du. Effect of overexpression of POLE2 on invasion and migration of non-small cell lung cancer cells[J]. Lingnan Modern Clinics in Surgery, 2020, 20(03): 307-310.

参考文献

[1] Bray F, Ferlay J, Soerjomataram I, et al.Global cancer statistics 2018:GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2018, 68(6):394-424.
[2] Siegel RL, Miller KD, Jemal A. Cancer Statistics, 2017[J]. CA Cancer J Clin, 2017, 67(1): 7-30.
[3] Chen Z, Fillmore CM, Hammerman PS, et al.Non-small-cell lung cancers:a heterogeneous set of diseases[J]. Nat Rev Cancer, 2014, 14(8): 535-546.
[4] Wood SL, Pernemalm M, Crosbie PA, et al.The role of the tumor-microenvironment in lung cancer-metastasis and its relationship to potential therapeutic targets[J]. Cancer Treat Rev, 2014, 40(4): 558-566.
[5] Miller KD, Nogueira L, Mariotto AB, et al.Cancer treatment and survivorship statistics, 2019[J]. CA Cancer J Clin, 2019, 69(5): 363-385.
[6] Spier I, Holzapfel S, Altmüller J, et al.Frequency and phenotypic spectrum of germline mutations in POLE and seven other polymerase genes in 266 patients with colorectal adenomas and carcinomas[J]. Int J Cancer, 2015, 137(2): 320-331.
[7] Frugoni F, Dobbs K, Felgentreff K, et al. A novel mutation in the POLE2 gene?causing combined immunodeficiency [J]. J Allergy Clin Immunol, 2016, 137(2): 635-638.e1.
[8] Hogg M, Johansson E.DNA polymerase ε[J]. Subcell Biochem, 2012, 62: 237-257.
[9] Zahurancik WJ, Baranovskiy AG, Tahirov TH, et al.Comparison of the kinetic parameters of the truncated catalytic subunit and holoenzyme of human DNA polymerase ?[J]. DNA Repair (Amst), 2015, 29: 16-22.
[10] Miyabe I, Kunkel TA, Carr AM.The major roles of DNA polymerases epsilon and delta at the eukaryotic replication fork are evolutionarily conserved[J]. PLoS Genet, 2011, 7(12): e1002407.
[11] Church DN, Stelloo E, Nout RA, et al.Prognostic significance of POLE proofreading mutations in endometrial cancer[J]. J Natl Cancer Inst, 2015, 107(1): 402.
[12] Meng B, Hoang LN, McIntyre JB, et al. POLE exonuclease domain mutation predicts long progression-free survival in grade 3 endometrioid carcinoma of the endometrium[J]. Gynecol Oncol, 2014, 134(1): 15-19.
[13] Gonzalez-Villasana V, Fuentes-Mattei E, Ivan C, et al.Rac1/Pak1/p38/MMP-2 Axis Regulates Angiogenesis in Ovarian Cancer[J]. Clin Cancer Res, 2015, 21(9): 2127-2137.
[14] Wang R, Ke ZF, Wang F, et al.GOLPH3 overexpression is closely correlated with poor prognosis in human non-small cell lung cancer and mediates its metastasis through upregulating MMP-2 and MMP-9[J]. Cell Physiol Biochem, 2015, 35(3): 969-982.
[15] Talvensaari-Mattila A, Paakko P, Turpeenniemi-Hujanen T.Matrix metalloproteinase-2 (MMP-2) is associated with survival in breast carcinoma[J]. Br J Cancer, 2003, 89(7): 1270-1275.
[16] Darlix A, Lamy PJ, Lopez-Crapez E, et al.Serum NSE, MMP-9 and HER2 extracellular domain are associated with brain metastases in metastatic breast cancer patients: predictive biomarkers for brain metastases?[J]. Int J Cancer, 2016, 139(10): 2299-2311.

POLE2对非小细胞肺癌细胞侵袭迁移能力的影响

Effect of overexpression of POLE2 on invasion and migration of non-small cell lung cancer cells

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	马晓武, 谭文亮, 杨磊, 谢智钦, 王庆斌, 陈亚进, 商昌珍. 环状RNA circKDM4B调控肝癌细胞增殖及侵袭迁移的实验研究[J]. 岭南现代临床外科, 2021, 21(06): 614-619.
[2]	钟启宇, 谢文练. circZBTB44通过调控AKT通路促进肾透明细胞癌增殖和迁移的研究[J]. 岭南现代临床外科, 2021, 21(03): 302-308.
[3]	黄贻培, 罗伟鑫, 许磊波. 转录因子YY1在肝细胞癌中的表达和功能研究[J]. 岭南现代临床外科, 2021, 21(01): 44-52.
[4]	朱畅, 傅宇翔, 夏利刚, 李方, 黄凯斌, 孙逍. 具核梭杆菌通过下调LCN2促进结直肠癌细胞增殖和迁移[J]. 岭南现代临床外科, 2020, 20(04): 411-415.
[5]	顾威庭, 赵俊文, 赵东明, 成侃, 蔡瑜. 微侵袭血肿清除术对高血压脑出血患者术后神经功能恢复及并发症的影响[J]. 岭南现代临床外科, 2020, 20(04): 463-467.
[6]	陈志良, 谢文练. circDOCK4促进肾透明细胞癌细胞增殖、迁移和侵袭的研究[J]. 岭南现代临床外科, 2020, 20(02): 139-145.
[7]	王雄1，王文己1，谢瑞敏2，李想2，王勇平1*. 过表达FOXO1对骨肉瘤细胞侵袭迁移能力的影响及机制研究[J]. 岭南现代临床外科, 2019, 19(03): 293-302.
[8]	胡学廷，姜明，郑泽华，林美贤，吴多光，王铭辉. 环状RNA circPRDM5在食管鳞状细胞癌中的表达和功能研究[J]. 岭南现代临床外科, 2019, 19(02): 139-144.
[9]	张瑞华，劳伟思，许英，蔡栋昊，毕卓菲，段朝晖*. LncRNA SNHG7通过调控β?catenin蛋白影响乳腺癌细胞的增值、迁移和侵袭[J]. 岭南现代临床外科, 2019, 19(01): 28-33.
[10]	施景龙罗兴喜许鹤洋蓝球生黄永亮褚忠华 . 肝再生磷酸酶-3上调转化生长因子-beta促进结肠癌LoVo细胞侵袭的机制研究[J]. 岭南现代临床外科, 2017, 17(03): 287-294.
[11]	林志立梁建平黄磊白植宝. EGFL7通过FAK信号促进舌鳞状细胞癌[J]. 岭南现代临床外科, 2016, 16(06): 660-663.
[12]	林在楷马保金. microRNA 与甲状腺癌侵袭转移关系的研究进展[J]. 岭南现代临床外科, 2016, 16(06): 747-750.
[13]	来伟施景龙林显敢许鹤洋蓝球生褚忠华. IL-6/p-AKT/p-STAT3信号通路在PRL-3促进结肠癌细胞增殖、迁移中的机制研究[J]. 岭南现代临床外科, 2016, 16(05): 517-520.
[14]	李红代亚娟黄少洁. MMP-2、PTEN、VEGF-C在大肠癌组织中的表达研究[J]. 岭南现代临床外科, 2016, 16(01): 44-47.
[15]	黄冠群罗捷周如建周新科简志祥. miRNA-144对肝癌侵袭性的影响[J]. 岭南现代临床外科, 2015, 15(02): 146-148.