关键词: 甲状腺乳头状癌, microRNA, 生物信息学, 外泌体

Abstract: ［Abstract］ Objective To investigate the exosomal microRNAs （miRNAs） expression in the postoperative drainage of papillary thyroid carcinoma （PTC） and nodular goiter and explore their role and mechanism in the development of PTC. Methods Exosomes were isolated from the postoperative drainage fluid of patients with PTC and nodular goiter on the first morning of thyroidectomy. Transmission electron microscope， particle size analysis and western blotting were used to characterize exosomes. And exosomal RNA was extracted and applied to miRNA microarray. The differently expressed miRNA was further analyzed by bioinformatics. Results Exosomes was identified in the perspective of morphology， size and protein markers. Exosomal miRNA profiles were significantly different between two groups and hsa?miR?609 had the greatest difference. Then the results of bioinformatics indicated that the genes predicted to be targeted by hsa?miR?609 were tied to the tumorgenesis of PTC. Conclusion The dysregulated exosomal miRNAs extracted from the postoperative drainage of PTC may play a regulatory role in the progress and development of PTC.

Key words: papillary thyroid carcinoma, exosomes, bioinformatics, microRNA