FOXP3 shRNA3对肝癌细胞趋化因子及受体CXCL12、CXCL11、CXCR4、CXCR7作用的研究

doi:10.3969/j.issn.1009-976X.2018.04.001

岭南现代临床外科 ›› 2018, Vol. 18 ›› Issue (04): 371-376.DOI: 10.3969/j.issn.1009-976X.2018.04.001

• 论著与临床研究 • 下一篇

FOXP3 shRNA3对肝癌细胞趋化因子及受体CXCL12、CXCL11、CXCR4、CXCR7作用的研究

欧希1，张光涛1，田佩凯2，陈景森3，徐喆4，谢勇1，王爱红1，刘吉奎1，刘晓平1*

1. 北京大学深圳医院肝胆胰外科，广东深圳 518036；2. 深圳大学总医院肝胆外科，广东深圳 518055；3. 深圳市妇幼保健院乳腺科，广东深圳 518028；4. 深圳市第三人民医院肝病三区，广东深圳 518112

通讯作者: 刘晓平

The silencing of forkhead box P3 gene inhibited expression of chemokines CXCL12，CXCL11， CXCR4 and CXCR7 in liver cancer cells

OU Xi1，ZHANG Guangtao1，TIAN Peikai2，CHEN Jingsen3，XU Zhe4，XIE Yong1，WANG Aihong1， LIU Jikui1, LIU Xiaoping1*

1. Department of Hepatopancreatobiliary Surgery， Peking University Shenzhen Hospital， Shenzhen， 518036， China； 2. Department of Hepatobiliary Surgery， Shenzhen University General Hospital， Shenzhen，518055，China；3. Breast Department，Shenzhen Maternity and Child Healthcare Hospital， Shenzhen，518028，China；4. The Third Ward Of Liver Disease，The Third People s Hospital of Shenzhen，Shenzhen 518112，China

Online:2018-08-20 Published:2018-08-20
Contact: Liu Xiaoping

摘要/Abstract

摘要： 【摘要】 [l2]目的研究FOXP3 shRNA对肝癌细胞株SMMC-7721和MHCC-97H的趋化因子及受体CXCL12、CXCL11、CXCR4、CXCR7的影响。方法设计三种编码FOXP3 shRNA的FOXP3干扰慢病毒: sh-FOXP3-1-pGreenPuro、sh-FOXP3-2-pGreenPuro和sh-FOXP3- pgreenpuro并分别转染SMMC-7721和MHCC-97H细胞。q-PCR检测各组CXCL12、CXCL11、CXCR4、CXCR7 mRNA的表达情况；Western Blot检测各组CXCL12、CXCL11、CXCR4、CXCR7蛋白的表达情况。结果经菌落PCR和测序验证证明三个FOXP3干扰慢病毒载体构建正确；三种干扰序列中sh-FOXP3-1干扰效果最明显，因此后期实验都使用sh-FOXP3-1进行下面的实验。研究显示：1、与对照组相比，sh-FOXP3-1组的CXCL12、CXCL11、CXCR4、CXCR7 mRNA表达明显下降，差异具有统计学意义。2、与对照组相比，sh-FOXP3-1组的CXCL12、CXCL11、CXCR4、CXCR7蛋白表达明显下降，差异具有统计学意义。结论 [l3] 干扰FOXP3的表达后，能减少趋化因子CXCL12、CXCL11、CXCR4、CXCR7的表达。

关键词: FOXP3, 趋化因子, 肝癌细胞, 趋化因子受体, shRNA

Abstract: ［Abstract］ Objective To study the effects of forkhead box P3 （FOXP3） shRNA on chemokine/ chemokine receptor CXCL12 、 CXCL11 、 CXCR4 、 CXCR7 expression in liver cancer cells. Methods Two hepatoma cell lines SMMC-7721 and MHCC-97H cells were selected. Three FOXP3 interfering lentivirus encoding FOXP3 shRNA were designed：sh?FOXP3?1?pGreenPuro，sh?FOXP3?2?pGreenPuro and sh?FOXP3?3?pGreenPuro. SMMC?7721 and MHCC?97H cells were transfected with lentivirus vector encoding shRNA-FOXP3 respectively. q-PCR was used to detect the mRNA expression of FOXP3，of CXCL12，CXCL11，CXCR4 and CXCR7 at the protein levels． Results Three FOXP3 interfering lentivirus carriers were constructed accordingly，and the effect of sh?FOXP3?1 was the most obvious in reducing FOXP3 expression. The following experiments were carried out using sh-FOXP3-1. The expression of CXCL12，CXCL11，CXCR4 and CXCR7 at both mRNA and protein levels in sh?FOXP3?1 group decreased significantly compared with controls. Conclusion The silencing of FOXP3 can inhibit the expression of chemokine / chemokine receptor CXCL12，CXCL11，CXCR4 and CXCR7.

Key words: FOXP3, liver cancer cells, chemokine receptor, shRNA, chemokine

中图分类号:

R681.5

欧希1，张光涛1，田佩凯2，陈景森3，徐喆4，谢勇1，王爱红1，刘吉奎1，刘晓平1*. FOXP3 shRNA3对肝癌细胞趋化因子及受体CXCL12、CXCL11、CXCR4、CXCR7作用的研究[J]. 岭南现代临床外科, 2018, 18(04): 371-376.

OU Xi1，ZHANG Guangtao1，TIAN Peikai2，CHEN Jingsen3，XU Zhe4，XIE Yong1，WANG Aihong1， LIU Jikui1, LIU Xiaoping1*. The silencing of forkhead box P3 gene inhibited expression of chemokines CXCL12，CXCL11， CXCR4 and CXCR7 in liver cancer cells[J]. Lingnan Modern Clinics in Surgery, 2018, 18(04): 371-376.

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FOXP3 shRNA3对肝癌细胞趋化因子及受体CXCL12、CXCL11、CXCR4、CXCR7作用的研究

The silencing of forkhead box P3 gene inhibited expression of chemokines CXCL12，CXCL11， CXCR4 and CXCR7 in liver cancer cells

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