关键词: 草酸钙, 泌尿系结石, 高血糖

Abstract: Objective To explorethe mechanism of hyperglycemia promoting the formation of calcium oxalate stones through cell and animal experiments. Methods Eight?week?old Wistar male rats （40 rats in total）were randomized into 4 groups：negative control group，hyperoxaluria model group， hyperglycemic model group，hyperglycemia group with high oxalic acid urine model group；10 rats in each group. The mRNA levels of the OPN，MCP?1，Cbfa1 and BMP?2 were analyzed with real?time reverse transcription polymerase chain reaction. The protein levels of OPN and MCP?1 in culture medium of renal tubular epithelial cells were detected by enzyme ? linked immunosorbent adsorption assay. Detecting calcium oxalate stones in renal tissue of rats from each group by calcium salt staining. Detecting the apoptosis of renal tubular epithelial cells in each group by apoptosis test. Detecting the expression of OPNin rat kidney tissues from each groupby immunohistochemistry. Detecting the expression ofkidney tissues from each groupby immunohistochemistry. Detecting the expression of MCP?1 in rats 24 hours urine from each group by enzyme linked immunosorbent adsorption assay. Results PCR and enzyme linked immunosorbent assay showed that OPN and MCP?1 were up?regulated in both mRNA and protein levels in high concentrations of glucose medium. Therewere large amounts of calcium salt deposition in renal tubules，large number of renal tubular epithelial cell apoptosis and OPN increased significantly in kidney tissues in hyperglycemia combined with hyperoxaluria model group，MCP?1 was increased in 24 hours urine from hyperglycemia combined with hyperoxaluria model group compare to other groups. Conclusion Hyperglycemia can induce the expression of chemokine，OPN and MCP?1，in renal tubular epithelial cells. Underhyperoxaluria，hyperglycemia can lead to the increase of apoptosis of renal tubular epithelial cells，and the formation of calcium oxalate stone increases. Hyperglycemia may contribute to the formation of calcium oxalate stones by increasing the local inflammatory response.

Key words: calcium oxalate, urinary calculi, hyperglycemia