关键词: P53突变, 结肠癌, KLF5

Abstract:

Objective  To investigate P53 mutation impact on the function and expression of KLF5 in colon cancer cell. Methods  Take the colon cancer cell lines RKO as the research object, which was both lower KLF5 expression and P53 wild type, chosen from the common colon cancer cells by Western blot and Sanger sequencing. Construct the over-expression/knock-down cell RKO-PCDNA-KLF5 and RKO-PCDNA-shKLF5, RKO-P53R175H-KLF5 and RKO-P53R175H-shKLF5 by the plasmid and verified by Western blot. Transwell assay was used to examine the invasion and metastasis ability. MTS assay was used to examine the proliferation ability. Results In the colon cancer cell lines RKO which was P53 wild type, Transwell assays revealed that the migratory and invasive capacities were inhibited by over-expression of KLF5, as well proliferation ability. And when the KLF5 was silent, the capacities of migratory and invasive, as well as proliferation ability were enhanced obviously. On the contrast with this result, the capacities of migratory, invasive and proliferation in the RKO-P53R175H cell with over-expres-sion KLF5 or silent KLF5 showed the opposite Conclusion. At the same time, the protein level expression of KLF5 in the RKO-P53R175H cell was declined, contrast to the RKO cell with P53 wild type. Conclu-sion In colon cancer cell lines RKO with high expression of P53 mutation, the protein expression of KLF5 levels dropped, and the gene function transformed from a tumor suppressor to the tumor promoter.

Key words: KLF5 , colon cancer, P53 mutation