关键词: 糖酵解, 结肠癌 , NDRG2

Abstract:

Objective To investigate whether tumor suppressor N-Myc downstream regulated gene 2（NDRG2）participates in regulating glucose metabolism in colon tumor cells. Methods NDRG2 was stably transfected in colon cancer cells HCT116. Lactate production was measured by lactate assay kits, and glucose consumption was measured by glucose assay kit. Besides, western blot was used to examine the expression of PKM、LDHA、HK, as the key glycolytic enzymes. Scratch test was used to test tumor cells proliferation. Results HCT116 which transfected with NDRG2 produced less lactate compared with control（38.2 ± 3.4 mmol/mL vs 62.1 ± 4.8 mmol/mL, P<0.05）, and remaining glucose was more than the control（137 ± 3.6 mmol/mL vs 88.2 ± 2.2 mmol/ml, P<0.05）. Western blot showed the expression of PKM、LDHA、HK were less than that in control. Scratch test showed less proliferation of colon tumor cells when above protein were inhibited. Conclusion NDRG2 functions to inhibits glycolysis of colon cancer cells as tumor suppressor gene by repressing the glycolytic enzymes expression, and then inhibits tumor cells proliferation.

Key words: NDRG2, colon cancer, glycolysis