关键词: 细胞粘附因子, 脐带静脉内皮细胞, 肝癌细胞, 利多卡因, NF-κB通路

Abstract: 【Abstract】 Objective To explore the effect of lidocaine on endothelial cell adhesion molecules. Methods Human umbilical vein endothelial cells （HUVECs） were preincubated with different concentration of lidocaine prior to tumor necrosis factor（TNFα, 10 ng?ml-1）for different time. Tumour cell adhesion assays were performed. The mRNA of endothelial-selectin （E-Selectin, CD62E）, intercellular adhesion molecule-1（ICAM-1）and vascular cell adhesion molecule-1（VCAM-1）were detected via quantitative reverse-transcriptase polymerase chain reaction（qRT-PCR）. The proteins of p65 were evaluated via western blotting. Results Compared with TNFα, the adhesion of HepG2 was significantly inhibited by lidocaine. CD62E, VCAM-1 and ICAM-1 expression were significantly promoted by TNFα. After pretreatment of lidocaine for 30 min, CD62E, VCAM-1 and ICAM-1 expression were significantly inhibited. Meanwhile, the phosphorylation of p65 were significantly attenuated by lidocaine. Conclusion Our results suggested that lidocaine modulated the expression of endothelial cell adhesion molecules and inhibited the adhesion of cancer cell.

Key words: Lidocaine, Hepatoma carcinoma cell, Cell adhesion molecules, NF-Kappa B pathway, Human umbilical vein endothelial cells