关键词: 微小RNA-150, 肝细胞癌, 预后

Abstract: 【Abstract】〓Objectives〓To investigate the expression and clinical significance of miR-150 in hepatocellular carcinoma(HCC). Methods〓miR-150 expression in liver cancer and paired normal tumor-adjacent tissues was determined by quantitative Real-time PCR (qRT-PCR). The expression difference between HCC and tumor-adjacent tissues was further verified using in situ hybrization(ISH) technique. Association of miR-150 expression in HCC tissues with clinicopathological status was examined by correlation analysis. Prognostic significance of miR-150 expression in HCC samples was detected with Kaplan-Meier method and Cox regression analysis. Results〓qRT-PCR assay showed 8/12 HCC cases had lower miR-150 expression compared with tumor-adjacent tissues and miR-150 expression level in HCC tissues markedly decreased (P=0.034). Similar to qRT-PCR results, ISH technique demonstrated most HCC specimens had negative or weak staining of miR-150, and staining intensity in 62% tumor-adjacent tissues was much higher than that in paired HCC tissues (P<0.001). Correlation analysis suggested miR-150 expression was significantly negatively correlated with AFP level, tumor size, TNM stage, histological grade and intravascular thrombus (P<0.05). Postoperative overall survival (OS) of miR-150 positive group was much longer that of miR-150negative group (P=0.004, Kaplan-Meier method). Cox univariate regression analysis implicated low miR-150 level in HCC samples was an unfavorable prognostic indicator (P=0.005). Conclusion〓Low expression of miR-150 in HCC specimens is related with a more aggressive tumor phenotype and indicates poor prognosis for HCC patients.

Key words: MicroRNA-150, Hepatocellular carcinoma, Prognosis