关键词: PRL-3, LoVo, EGFR, MEK-ERK

Abstract: 【Abstract】〓Objective〓To observe protein tyrosine phosphatase -3 (PRL-3) related regulatory function on epidermal growth factor (EGFR) signaling pathway in colon cancer LoVo cells. Methods After PRL-3 was transfected to LoVo cells (hereinafter referred to as LoVo-P) for 48 h, phosphorylation of EGFR protein expression and phosphorylation of MEK1/2 (p-MEK1/2), Erk1/2 (p-Erk1/2), Msk1/2 (p-Msk1/2) from MEK-ERK pathway were detected by Western blot assay. Results〓After transfected PRL-3, LoVo cells activated MEK-ERK channel. LoVo-C had an ability to activate phosphorylation of EGFR and Western-Blot showed an increase of 72.0% when compare with control group LoVo cells. High PRL-3 expression also induced to expression of p-MEK1/2, p-Erk1/2, p-Msk1/2. Conclusion〓In colon cancer cells, PRL-3 involved EGFR signaling pathway regulation by activation of phosphorylation of EGFR.

Key words: PRL-3, LoVo, EGFR, MEK-ERK