关键词: 膀胱癌, 微小RNA, 迁移, 侵袭, EMT

Abstract:

【Abstract】〓Objective〓To study the effects of microRNA-155(miR-155) on migration and invasion of bladder cancer cell um-uc-3. Methods〓MiR-155 expression was detected in bladder cancer tissues. After exogenous transfection of miR-155 mimics into um-uc-3,.Transwell chamber assay and wound healing assay were performed to assess cell migration and invasion..Expression of EMT markers was evaluated by quantitative real-time PCR and western blotting. Results〓MiR-155 expression was higher in bladder cancer tissues than adjacent normal tissues...Over-expression of miR-155 promoted migration and invasion in bladder cancer cells of um-uc-3..Both mRNA and protein expressions of β-catenin, vimentin and snail were higher in miR-155 transfected cells, while claudin-1 showed no significance. Conclusion〓Over-expression of miR-155 can promote migration and invasion of bladder cancer cells with higher expressed EMT markers like β-catenin, vimentin and snail.

Key words: microRNAs, Bladder cancer, Migration, Invasion, EMT