关键词: 前列腺癌, 自然杀伤T细胞, 细胞因子, 免疫调节

Abstract: 【Abstract】〓Objective〓To observe the changes and significance of natural killer T cells for the patients with prostate cancer undergoing extraperitoneal laparoscopic radical prostatectomy(ELRP). Methods〓Thirty-five patients with prostate cancer and 35 healthy subjects were enrolled. The peripheral blood of the two groups were collected, and the levels of NKT cells and its intracellular gamma interferon(IFN-γ) and interleukin 4 (IL-4) using flow cytometry test. Two weeks after ELRP, the prostate cancer patients underwent the second test of NKT and its cytokines. Results〓Compared with the healthy subjects,levels of NKT,IFN-γ and IL-4 belonging to the patients with prostate cancer were 5.92±1.47,.4.23±1.90 and 2.15±1.04, which were significantly lower than that of the healthy subjects (12.53±4.16, 8.94±2.76 and 5.86±1.97,.P<0.05). Levels of NKT, IFN-γ and IL-4 increased significantly after ELRP when compared with preoperation (9.13±2.69, 6.19±2.03 and 3.37±1.28, P<0.05),and the mean fluorescence intensity of IFN-γ increased more apparently than IL-4. Conclusion〓Decreasing of NKT, IFN-γ and IL-4 is an important immunological characteristic for patients with prostate cancer, and ELRP can improve the cellular immune dysfunction in patients with prostate cancer effectively.

Key words: Prostate cancer, Natural killer T cells, Cytokines, Immune regulation