关键词: 胃癌, CD4⁺CD29⁺T细胞, 细胞因子, 血管生成, 相关性分析

Abstract:

【Abstract】〓Objective〓To explore the expression and significance of CD4⁺CD29⁺T cells for patients with gastric carcinoma. Methods〓Sixty-four patients with gastric cancer, including Ⅰ-Ⅳ stage, were enrolled, as well 60 chronic gastritis patients and healthy subjects. The peripheral blood CD4⁺CD29⁺T cells and cytokines γ-interferon (IFN-γ) and interleukin 4 (IL-4) were tested using flow cytometry. The levels of intercellular adhesion molecule 1 (ICAM-1), transforming growth factor beta 1 (TGF-beta 1) and carcinoembryonic antigen (CEA) were also tested. The correlation between the above-mentioned 6 indexes was analyzed via Pearson test. Results〓The percentage of CD4⁺CD29⁺T cells and mean fluorescence intensity of IL-4 of the gastric cancer patients were higher than those in the chronic gastritis and healthy subjects (P<0.05); whereas the mean fluorescence intensity of IFN-γ were lower than the other two groups for the gastric cancer patients. CD4⁺CD29⁺T cells and IL-4 increased and but IFN-γ decreased for the Ⅰto Ⅳ stage gastric cancer patients, and the levels of ICAM-1,.TGF-β1and CEA also increased when compared with the other two groups (P<0.05). Pearson analysis showed that CD4⁺CD29⁺T cells and IL-4 positively correlate with CAM-1, TGF-β1 and CEA; but IFN-γ negatively correlate with CAM-1, TGF-β1 and CEA (P<0.05). Conclusion〓Increase of CD4⁺CD29⁺T cells and IL-4,.as well the decrease of IFN-γ were the important immunological features for gastric cancer patients. CD4⁺CD29⁺T cells, IL-4 and IFN-γ were differently expressed in early and end stages for patients with gastric carcinoma significantly, and they correlated with the factors which are closely related the tumor angiogenesis.

Key words: Gastric carcinoma, CD4⁺CD29⁺T cells, Cytokines, Angiogenesis, Correlation analysis