心搏骤停心肺复苏过程中大鼠脑皮质葡萄糖转运体3表达的变化

doi:j.issn.1009-976X.2013.02.005

摘要/Abstract

摘要：

【摘要】目的研究在心搏骤停心肺复苏过程中大鼠脑皮质葡萄糖转运体3(GLUT3)转录水平和蛋白表达水平的变化。方法 30只大鼠随机分为两组,假手术组(S,n=5),心肺复苏组(R,n=25),心肺复苏组分别选取自主循环恢复(ROSC)3、6、12、24、48 h 5个时间点为5个小组,用窒息法制备稳定的大鼠心搏骤停及心肺复苏模型。R组在ROSC 3、6、12、24、48 h取大脑双侧顶叶皮质,分别用实时荧光定量PCR及免疫组织化学半定量方法(SP法)检测相应时间点GLUT3mRNA和蛋白的表达。结果〓R组与S组相比较,GLUT3mRNA水平在ROSC3h出现轻微升高,在ROSC 6 h及24 h达高峰,12 h GLUT3mRNA表达水平出现一下降点,但仍高于S组,ROSC48h GLUT3mRNA仍高于假手术组。GLUT3蛋白表达的变化趋势与GLUT3mRNA的变化一致。结论在心搏骤停心肺复苏过程中,所造成的全脑缺氧缺血状态会使大鼠脑皮质GLUT3的表达水平上调,可能是机体对缺血损伤的保护性反应。

关键词: 心肺复苏, 大鼠, 脑;葡萄糖转运体3

Abstract:

【Abstract】 Objective To investigate changes of the expression of rat cortical GLUT3 mRNA and protein during cardiopulmonary resuscitation of cardiac arrest. Methods Thirty of SD rats were randomly divided into sham operation group(S group,n=5), resuscitation group (R group, n=25),and resuscitation group was further divided into five subgroups at restoration of spontaneous circulation(ROSC) 3,6,12,24,48 h. The rat model of asphyxial cardiac arrest and cardiopulmonary resuscitation (CPR) was established and the animals were sacrificed at each time point after CPR,and brain tissues were taken..Real-time qPCR and immunohistochemistry staining were performed to evaluate cortical GLUT3 expression of parietal lobe. Results In R group, GLUT3 increased gradually at ROSC 3 h,peaked at ROSC6 and 24 h, while dropped at ROSC 12 h. An upregulation of GLUT3 still existed at ROSC 24 h,although dropped at ROSC 12 h. Conclusion GLUT3 expression increased after cerebral ischemia and hypoxia caused by cardiac arrest and CPR, which may be a protective response to ischemic injury.

Key words: Cardiopulmonary resuscitation, Rat, Brain, Glucose transporter 3

中图分类号:

R602

陈瑞霞叶西就. 心搏骤停心肺复苏过程中大鼠脑皮质葡萄糖转运体3表达的变化[J]. 岭南现代临床外科, 2013, 13(02): 95-98.

Chen Ruixia; Ye Xijiu. Changes of rat cortical GLUT3 expression during cardiopulmonary resuscitation[J]. Lingnan Modern Clinics in Surgery, 2013, 13(02): 95-98.

参考文献

1. Naqamatu S,et al.Gene expression of GLUT3 glucose transporter regulated by glucose in vivo in mouse brain and in intro in neuronal cell culture from embryos.Biochem J,1994,300:125-31.
2. Vannucci SJ.Glucose transporter expression in brain:relationship to cerebral glucose utilization.Dev Neurosci,1998,20:3689-79.
3. I.Stuart W, Paul T. Glucose transporters(GLUT and SGLT): expanded families of sugar transporter proteins. British Journal of Nutrition, 2003,89:3-9.
4. Vannucci SJ, Maher F, Simpson IA.Glucose transporter proteins in brain: delivery of glucose to neurons and glia.GLIA, 1997, 21:2-21.
5. Yu S.Hypoxic preconditioning up-regulateds glucose transport activity and glucose transporter(GLUT1 and GLUT3) gene expression after acute anoxic exposure in the cultured rat hippocampal and astrocytes .Brain Res,2008,1211:22-29.
6. Vannucci SJ , et al. Alterations in GLUT1 and GLUT3 glucose transporter gene expression following unilateral hypoxia ischemia in the immature rat brain.Brain Res Dev Brain Res,1998,107:2255-64.
7. 李方成等.葡萄糖转运体3在脑缺血/再灌注后半影区表达的变化及意义.中国病理生理杂志, 2004,20:2276-9.
8. Hendriekx HHL，Rao GR，Safar P，et al. Asphyxia cardiac arrest and resuscitation in rats. Resuscitation, 1984, 12(2):97-11
9. American Heart Association. Utstein-Style Guidelines for Uniform Reporting of Laboratory CPR Research. 1996,94:2324-2336.
10.Buunk G, van der Hoeven JG, Meinders AE. Cerebral blood ?ow after cardiac arrest. Neth J Med 2000;57(3):106—12.
11.Nishizawa H, Kudoh I. Cerebral autoregulation is impaired inpatients resuscitated form cardiac arrest. Acta Anaesthesiol Scand 1996;40(9):1149—53.
12.Sundgreen C, Larsen FS, Herzoq TM, Knudsen GM, Boesgaard S,Aldershville J. Autoregulation of cerebral blood ?ow in patients resuscitated from cardiac arrest. Stroke 2001;32(1):128—32.
13. Shaffner DH, Eleff SM, Koehler RC, Traystman RJ. Effect of the no-?ow interval and hypothermia on cerebral blood ?ow and metabolism during cardiopulmonary resuscitation in dogs.Stroke 1998;29(12):2607—15.
14. Virginie L, Olivier H, Bernard V, et al. Changes in cerebral blood flow and oxygen extraction during post-resuscitation syndrom. Resuscitation, 2008, 76:17-24.
15. Virginie L, Olivier H, Bernard V, et al. Changes in cerebral blood flow and oxygen extraction during post –resuscitation syndrome. Resuscitation, 2008，76：17-24.
16. Edgren E, Enblad P, Grenvik A, et al. Cerebral blood ?ow and metabolism after cardiopulmonary resuscitation. A pathophysiologic and prognostic positron emission tomography pilot study.Resuscitation 2003;57(2):161—70.
17. William D, F. Micchelle L. B, Kevin M. B, et al. Hypoxic-ischemic brain injury(HIBI) after cardiopulmonary arrest. Current Anaesthesia & Critical Care, 2007, 18:261-276.
18. Sherin U.D, Premeela A. R, Richard B.M, et al. Effect of development and hypoxic-ischemia upon rabbit brain glucose transporter expression. Brain Research, 1999, 823(113-128)
19. Gerhart DZ, Leino RL, Taylor WE,et al. GLUT1 and GLUT3 gene expression in gerbil brain following brief ischemia: an in situ hybridization study. Brain Res Mol Brain Res. 1994: 25(3-4):312-22.
20. Espinoza-Rojo M, Iturralde-Rodriguez KI, Aguilera P, et al. Glucose transporters regulation on ischemia brain: possible role as therapeutic target.Cent Nerv SystAgents Med Chem, 2010, 10(4):317-25.
21. Bergeron M, Yu AY, Solway KE, et al. Induction of hypoxia-inducible factor (HIF-1)and its target genes following focal ischemia in rat brain. Eur J Neurosci, 1999, 11(12): 4159-4170.
22. D’ery MA, Michaud MD, Richard DE. Hypoxia-inducible factor 1: regulation by hypoxic and non-hypoxic activators. The International Journal of Biochemistry & Cell Biology, 2005, 37(3): 535–540.
23. Munell F, Burke RE, Bandele A, Gubits RM. Localization of c-fos, c-jun, and hsp70 mRNA expression in brain after neonatal hypoxia-ischemia. Brain Res,1994, 77(1): 111-121.
24. Sharp FR, Ran R, Lu AG, et al. Hypoxic preconditioning protects against ischemic brain injury. NeuroRx, 2004, 1(1): 26-35.

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