关键词: 膀胱, 上皮细胞, 细胞培养, 组织贴块法, 酶消化法

Abstract: Objective Bladder transitional epithelial cells from rabbit were primary cultured by tissue explant and enzyme digestion method respectively to explore the better culture technique in vitro and provide seed cells for engineering of bladder tissue. Methods Rabbit bladder transitional epithelial cells were primary cultured by tissue explant and enzyme digestion method respectively. The cell morphology and proliferation characteristics were oberserved. The grow curve of the cells was described through cell counting.The cells were identified by cell morphology and immunofluorescence. Results The bladder transitional epithelial cells were obtained by the two methods possessed a typical morphological feature of epithelial cells. And the morphology and growth characteristics were essentially consistent. The growth curve of the cells was similar to “S”shape. It showed vimentin negative and cytokeratin AE1/AE3 positive. Conclusion Both the culture methods could obtain bladder transitional epithelial cells highly purity. The method of tissue explant has a higher success rate but spending more time than that of enzymatic digestion.

Key words: Bladder, Epithelial cells, Cell culture, Tissue explant, Enzyme digestion method