关键词: 大肠癌, STAT3信号通路, RNA干扰, shRNA

Abstract: 【Abstract】 Objective To investigate the effects of STAT3 signaling pathway on cells proliferation and VEGF expressing in human colorectal cancer HT-29 cells. Methods HT-29 cells were transfected with the pRNAT-shSTAT3 recombinant plasmids. Real time quantitative PCR and Western-blot were used to quantify the expression of STAT3 gene. The cell growth was assessed by MTT assay. VEGF expression was detected by Real time quantitative PCR and ELISA. Results STAT3 shRNA could inhibit the expression of STAT3 gene in HT-29 cells. In the transfected group with the recombinant plasmid, the proliferation of HT-29 cells was suppressed and VEGF expression was inhibited, which was significantly different from that of the control group(р＜0.01).Conclusions STAT3 singling pathway in human colorectal cancer cells can inhibit cells proliferation and VEGF expression greatly.