关键词: 调节性T细胞, 细胞因子, 慢性前列腺炎, 免疫机制, 单核细胞, Regulatory T cells, Cytokines, Chronic abacterial prostatitis, Immune mechanism, Monocyte

Abstract: Objective To explore the expression and its significance of CD4+CD25+ regulatory T cells (Tr) in pathogenesis of chronic abacterial prostatitis patients (CAP). Methods 20 CAP patients and 10 volunteers were taken into this study and their peripheral blood was collected for testing the Tr through flow cytometry. Foxp3, the important regulatory factor of Tr, was detected by RT-PCR. The Tr’ cytokines including IL-10 and TGF-β1 in both peripheral blood (PB) and prostatic fluid(PF) were measured by ELISA. Routine blood tests (BT), C-reaction protein (CRP) and erythrocyte sedimentation rate (ESR) were also compared as the inflammatory indexes. Results Compared with the volunteers, percentage of Tr in the CAP patients was significantly lower so was the Foxp3 (P=0.013 and 0.000 respectively). About the cytokines, the IL-10 and TGF-β1 in both PB and PF of the CAP group were significantly lower than that in the volunteers, so was the monocyte in PB (MONO). But there was no statistically difference of WBC, NEU, LYM, CRP and ESR between the two groups. Pearson correlation analysis showed that MONO correlated with the quantities of Tr, Foxp3, IL-10 and TGF-β1. Conclusion Decreased Tr resulting insufficient of immunodepression and degressive IL-10 causing proliferation of MONO, which leaded to the excessive antigen presenting and immune injury, maybe a key role in pathogenesis of chronic abacterial prostatitis.