关键词: 金黄色葡萄球菌, Panton-Valentine杀白细胞素, 耐甲氧西林金黄色葡萄球菌, C反应蛋白, 中性粒细胞与淋巴细胞比值

Abstract: Objective To understand the prevalence and resistance of Staphylococcus aureus （S. aureus）carrying the Panton-Valentine leukocidin （PVL） gene in surgical patients at the Shandong Provincial Hospital Affiliated to Shandong First Medical University in 2023. The clinical application values of white blood cell （WBC）, C-reactive protein （CRP）, and neutrophil-to-lymphocyte ratio （NLR） in the diagnosis of PVL-positive S. aureus infection were investigated. Methods 100 strains of S. aureus were isolated. The presence of the mecA gene and genes encoding PVL were ditermined using PCR, and the drug resistance of the isolates were analyzed. Groups were grouped according to the mecA and PVL gene test results. Contemporaneous 100 subjects undergoing healthy physical examination were selected as the control group. The levels of WBC, CRP, and NLR of each group were collected, and the clinical application values of WBC, CRP, and NLR for PVL-positive S. aureus infection were compared. Results Among the 100 strains of S. aureus, 47 isolates were Methicillin-resistant Staphylococcus aureus（MRSA）. Of the 49 S. aureus strains tested, the PVL gene was detected in most of them, which were mainly methicillin-sensitive Staphylococcus aureus （MSSA）. The levels of WBC, CRP, and NLR increased significantly during S. aureus infection, and the level of NLR in the PVL-positive group was significantly higher than that in the PVL-negative group （Z=-2.26, P=0.02）. Still, the levels of WBC, and CRP were rarely different between the PVL-positive group and PVL-negative group. Conclusion Compared with WBC and CRP, NLR had a higher clinical application value for PVL-positive S. aureus, and NLR can be used as a predictor of PVL-positive S. aureus infection.

Key words: Staphylococcus aureus, Panton-Valentine leucocidin, Methicillin-resistant Staphylococcus aureus, C-reactive protein, Neutrophil-to-lymphocyte ratio