关键词: 环境因素, 医用多酶清洗剂, 酶活性

Abstract: Objective To detect the activity of enzymes in the working solution at different temperature at a certain time interval, so as to determine the best use conditions of the common multi-enzyme medical detergent. Methods Four kinds of multi-enzyme medical detergent from different manufacturers were selected. Solution A was the fast and efficient multi-enzyme medical detergent. Solution B is omnipotent multi-enzyme medical detergent. Solution C is specific alkaline multi-enzyme medical detergent. Solution D is full effect multi-enzyme medical detergent. At room temperature, A, B, C and D were diluted 200 times with pure water to form the working solution, and the pH value of the working liquid was determined. Four different multi-enzyme medical detergent working solution A, B, C and D were placed in a water bath at 15 ℃, 20 ℃, 25 ℃ and 30 ℃ at constant temperature, and stored at different intervals of 1 h, 3 h, 6 h and 8 h. The enzyme activities in the working solutions were measured by sampling, and the enzyme activity stability curve was drawn. Results Under the condition of 15℃~20℃, only about 10% enzyme activity was decreased in 8 h. Under the condition of the same loss, only at 6 h the enzyme activity could be maintained at 25 ℃, and at 3 h the enzyme activity could be maintained at 30 ℃. Conclusion It is suggested that the multi-enzyme medical detergent can be used within 3 h at room temperature after being diluted into working solution. Or under the condition of refrigeration, used within 8 h. The full effect multi-enzyme medical detergent showed the higher enzyme activity stability.

Key words: environmental factors, multi-enzyme medical detergent, enzyme activity