MiR-130b-3p在非小细胞肺癌患者血液中的表达及潜在意义

doi:10.3969/j.issn.1009-976X.2022.06.004

岭南现代临床外科 ›› 2022, Vol. 22 ›› Issue (06): 547-554.DOI: 10.3969/j.issn.1009-976X.2022.06.004

MiR-130b-3p在非小细胞肺癌患者血液中的表达及潜在意义

李靖霄¹, 李国盛¹, 徐阳¹, 何融泉², 周华富³, 孔晋亮⁴, 陈罡^1,*

1.广西医科大学第一附属医院病理科,南宁 530021;
2.广西医科大学第一附属医院肿瘤内科,南宁 530021;
3.广西医科大学第一附属医院心胸外科,南宁 530021;
4.广西医科大学第一附属医院呼吸内科,南宁 530021

通讯作者: * 陈罡,Email：chengang@gxmu.edu.cn
基金资助:
广西医疗卫生适宜技术开发与推广应用项目（S2020031）; 广西教育科学规划2021年度重点课题B类（2021B167）

The expression of miR-130b-3p in the blood of patients with non-small cell lung cancer and its potential significance

LI Jing-xiao¹, LI Guo-sheng¹, XU Yang¹, HE Rong-quan², ZHOU Hua-fu³, KONG Jin-liang⁴, CHEN Gang¹

1. Department of Pathology, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China;
2. Department of Oncology, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China;
3. Department of Cardiothoracic Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China;
4. Department of Respiratory Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China

Received:2022-03-17 Online:2022-12-20 Published:2023-02-03
Contact: CHEN Gang, chengang@gxmu.edu.cn

摘要/Abstract

摘要： 目的探讨miR-130b-3p在NSCLC患者血液中的表达水平及潜在临床意义。方法本研究使用癌症基因组图谱（TCGA）、基因表达谱数据库（GEO）以及序列片段归档数据库（SRA）下载了NSCLC相关的miRNAs表达谱,并提取miR-130b-3p在各个纳入的数据集中的表达量。计算标准化均数差（SMD）综合展示miR-130b-3p在NSCLC患者血液中的表达水平,进行诊断试验以探讨差异表达的miR-130b-3p对NSCLC患者和健康人的鉴别潜力。通过对miRwalk 2.0中12个数据库的预测结果进行交集,筛选出miR-130b-3p的下游靶基因,并采用富集分析探讨miR-130b-3p下游靶基因调控NSCLC的潜在信号通路。结果共7项研究的300例NSCLC患者血液样本被纳入。miR-130b-3p在LUAD（SMD=-1.45,95%CI：-2.66~-0.23）以及LUSC（SMD=-0.46,95%CI：-0.84~-0.09）这两种NSCLC患者血液中的表达水平较对照组明显下调。诊断试验提示miR-130b-3p具有良好的区分NSCLC患者和健康人的潜力（AUC=0.85：95%CI：0.82~0.88）。47个基因被鉴定为miR-130b-3p的下游靶基因,这些靶基因显著富集于Renin-angiotensin system通路。结论在NSCLC患者血液中表达下调的miR-130b-3p有望用于NSCLC的筛查。

关键词: 微小RNA, miR-130b-3p, 非小细胞肺癌, 血液

Abstract: Objective This research aims to explore the expression and potential clinical significance of miR-130b-3p in the blood of NSCLC patients. Methods The Cancer Genome Atlas, Gene Expression Omnibus, and Sequence Read Archive were used to download NSCLC-associated miRNAs expression data, then the expression data of miR-130b-3p was extracted from included datasets. Estimating standardized mean difference （SMD） to comprehensively present the expression level of miR-130b-3p in the blood of NSCLC patients. A diagnostic test was performed to explore the discriminated potential of differentially expressed miR-130b-3p between NSCLC patients and healthy people. By making an interaction for predicted results from 12 databases in miRwalk 2.0, we screened out the downstream target genes of miR-130b-3p. Enrichment analysis was used to find potential signaling pathways of miR-130b-3p downstream target genes in regulating NSCLC development. Results A total of 300 blood samples from seven studies were included. MiR-130b-3p is downregulated in blood of NSCLC patients, including both LUAD （SMD=-1.45,95%CI： -2.66~-0.23） and LUSC （SMD=-0.46, 95%CI：-0.84~-0.09）. The diagnostic test indicated miR-130b-3p has a good potential to discriminate NSCLC patients from healthy people AUC=0.85,95%CI：0.82~0.88）. Forty-seven genes are identified as miR-130b-3p downstream targets, they significantly enrich in Renin-angiotensin system pathway. Conclusion MiR-130b-3p is downregulated in the blood of NSCLC patients and is expected to be used for early screening of NSCLC.

Key words: microRNA, MiR-130b-3p, non-small cell lung cancer, blood

中图分类号:

R734.2

李靖霄, 李国盛, 徐阳, 何融泉, 周华富, 孔晋亮, 陈罡. MiR-130b-3p在非小细胞肺癌患者血液中的表达及潜在意义[J]. 岭南现代临床外科, 2022, 22(06): 547-554.

LI Jing-xiao, LI Guo-sheng, XU Yang, HE Rong-quan, ZHOU Hua-fu, KONG Jin-liang, CHEN Gang. The expression of miR-130b-3p in the blood of patients with non-small cell lung cancer and its potential significance[J]. Lingnan Modern Clinics In Surgery, 2022, 22(06): 547-554.

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MiR-130b-3p在非小细胞肺癌患者血液中的表达及潜在意义

The expression of miR-130b-3p in the blood of patients with non-small cell lung cancer and its potential significance

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