Luminal型乳腺癌胞膜蛋白相关预后模型的构建与验证

doi:10.3969/j.issn.1009-976X.2021.03.006

摘要/Abstract

摘要： 目的 Luminal型乳腺癌是最常见的一种乳腺癌分子亚型,构建有效的预后模型与寻找有效的治疗靶点对luminal型乳腺癌患者有重要的临床意义。胞膜蛋白（PMPs）在肿瘤的发生发展中发挥着重要作用,本研究着力于构建PMPs相关预后模型,并在此基础上探索可能的治疗靶点。方法从TCGA数据库中下载luminal型乳腺癌的转录组测序数据及临床信息作为训练集,通过筛选差异表达PMPs基因,运用预后相关基因构建PMPs预后模型。同时从GEO数据库中下载相关数据,以验证本研究中所构建模型的效能。结果在建构的预后模型中,ADRA1B、CD99L2、EZR、IYD、RGS9BP和SLC16A2基因高表达与预后负相关,而DUS1L、KIT、MS4A1、PI3和SUSD2基因的表达则与预后正相关。通过所构建的模型计算出各患者的风险评分,并结合其总生存时间和生存状态,发现高风险组患者的预后均较差。结论从PMPs预后基因中筛选出11个可用于建立luminal型乳腺癌预后模型的最佳基因,这个模型可能为临床上luminal型乳腺癌患者提供有效的预后预测。

关键词: 胞膜蛋白, luminal型乳腺癌, 预后模型, TCGA, GEO

Abstract: Objective Plasma membrane proteins （PMPs） play an indispensable role in tumor development and progression. Luminal breast cancer is the most common molecular subtype of breast cancer seriously threatening the health of women, which makes it crucial to construct a more accurate prognostic model and find out precise therapeutic targets to improve the prognosis for luminal breast cancer patients. Methods We downloaded conforming transcriptome RNA-sequencing data and their clinical information about luminal breast cancer from TCGA database as a training set. After identifying differentially expressed plasma membrane proteins-related genes, we screened out the prognostic genes for further analysis. And we downloaded relevant data from GEO database and processed them as a validation set. Results A prognostic model was constructed. In this optimal prognostic model, ADRA1B, CD99L2, EZR, IYD, RGS9BP, and SLC16A2 were associated with high risk, while DUS1L, KIT, MS4A1, PI3, and SUSD2 were correlated with low risk. By calculating the risk score and combining respective overall survival time and survival status, we found that the high-risk group had statistically worse prognosis than the low-risk one. Conclusion Eleven optimal genes from PMPs-related prognostic genes to establish and validate a prognostic model for luminal breast cancer was screened out, which may be an effective and useful prognostic model for patients with luminal breast cancer.

Key words: plasma membrane proteins, prognostic model, luminal breast cancer, TCGA, GEO

中图分类号:

R737.9

朱钊雯, 贾卫娟, 刘洁琼. Luminal型乳腺癌胞膜蛋白相关预后模型的构建与验证[J]. 岭南现代临床外科, 2021, 21(03): 281-286.

ZHU Zhao-wen, JIA Wei-juan, LIU Jie-qiong. A novel plasma membrane proteins-related prognostic model for luminal breast cancer[J]. Lingnan Modern Clinics In Surgery, 2021, 21(03): 281-286.

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