关键词: 骨髓间充质干细胞, 成骨分化, PI3K-AKT, TLN1

Abstract: ［Abstract］ Objective To investigate the role and regulatory mechanism of Talin 1 in osteogenic differentiation of bone marrow mesenchymal stem cells. Methods The expression level of TLN1 in osteogenic differentiation of mesenchymal stem cells was detected by qRT-PCR and western blot. The proliferation of MSC was measured by CCK-8 assay after TLN1-siRNA intervention. The osteogenic differentiation capacity was determined through alizarin red S and alkaline phosphatase assays. Western blotting was used to study the molecular mechanism of TLN1 in the osteogenic differentiation of MSC. Results qRT-PCR and Western blot results suggested that TLN1 expression increased during osteogenic differentiation. The osteogenic differentiation capacity was decrease compared with the negative group after siRNA transfection. After transfection with siRNA， the expression of p-AKT was decreased by Western blot. Furthermore， the rescue experiment showed that active PI3K/AKT pathway can reserve osteogenic differentiation capacity. Conclusion Talin1 expression was gradually up-regulated after induced osteogenic differentiation of MSC. TLN1 knockdown inhibited osteogenic differentiation of MSC through PI3K/AKT pathway.

Key words: TLN1, PI3K/AKT pathway, osteogenesis, mesenchymal stem cells