关键词: 去泛素化酶3, 泛素化, 前列腺癌, 上皮间质化

Abstract: ［Abstract］ Objective To explore the expression of Deubiquitinase 3 （DUB3） in different human prostate cancer cell lines and the effect on cell proliferation， migration and epithelial?mesenchymal transition in prostate cancer. Methods Western Blot and quantitative RT?PCR were performed to detect the protein and mRNA expression of DUB3 in prostate cancer cell lines and normal prostate epithelial cell line. Then， PC?3 cells were transiently transfected with siRNA for DUB3 silencing， and MTS and Transwell were performed to measure the cell proliferation and migration. Finally， we used Western Blot to investigate the relationship between DUB3 and Snail1. Results The results of Western Blot and qRT?PCR showed that both the protein and mRNA levels of DUB3 were obviously higher in prostate cancer cell lines than in normal prostate epithelial cell line （P<0.05）|After DUB3 was inhibited by siRNA， the cell proliferation and cell migration of PC?3 were both obviously decreased （P<0.05）. Furthermore，the expression of Snail1 in prostate cancer cells was also downregulated. Conclusion DUB3 is overexpressed in prostate cancer and promotes cells proliferation and migration. Importantly， DUB3 may facilitate EMT in prostate cancer by deubiquitination of Snail1. USP3 is expected to be a candidate target for prostate cancer treatment.

Key words: ubiquitination, deubiquitinase 3, prostate cancer, epithelial?mesenchymal transition