关键词: 骨肉瘤, 侵袭, 迁移, FOXO1

Abstract: ［Abstract］ Objective To investigate the effect of FOXO1 on invasion and migration of osteosarcoma cells. Methods Real?time quantitative PCR （qRT?PCR） was used to detect the mRNA expression level of FOXO1 in human osteoblast hFOB 1.19 and human osteosarcoma cells U2OS and MNNG/HOS. The FOXO1 overexpressed cell model was established by the recombinant plasmid of pcDNA3.1?FOXO1， and the empty plasmid （pcDNA3.1?vector） was used as a control. The transfection efficiency was verified by the method of qRT?PCR and Western blot.The proliferation ability （absorbance value） of osteosarcoma cells was detected by Cell Counting Kit?8 （CCK?8） test after the successful overexpression of FOXO1 gene. The invasion and migration ability of osteosarcoma cells was detected in Transwell experiment in vitro， and the matrix metalloproteinase 9 was detected by qRT?PCR and western blot methods. Results The expression level of FOXO1 mRNA in MNNG/HOS and U2OS of human osteosarcoma cells was significantly lower than that of human osteoblast hFOB 1.19 （P<0.05）， and the expression level of FOXO1 in osteosarcoma cell U2OS was much lower. After transfection of recombinant plasmid pcDNA3.1?FOXO1， the expression level of FOXO1 in osteosarcoma cell U2OS increased significantly. Compared with the control group （pcDNA3.1?vector）， the proliferation of U2OS?FOXO1 overexpressed cells decreased （P<0.05）， and the ability of invasion and migration and migration decreased， and the expression level of MMP9 was significantly lower than that of the control group （P<0.05）. Conclusion The expression level of FOXO1 in osteosarcoma cells is significantly lower than that in normal osteoblasts. Overexpression of FOXO1 may inhibit the invasion and migration of osteosarcoma cells by downregulating MMP9.

Key words: osteosarcoma, FOXO1, migration, invasion