关键词: 骨髓间充质干细胞, 前列腺素E1, 凋亡, 浓度

Abstract: ［Abstract］ Objective To explore the optimal concentration of prostaglandin E1 against apoptosis of rat bone marrow mesenchymal stem cells in vitro. Methods Bone marrow mesenchymal stem cells were extracted from SD rats, rat bone marrow mesenchymal stem cells （P3?P5） were cultured in hypoxia/ serum?deprivation（H/SD）model, in vitro, and Prostaglandin E1 take different concentrations（0 μg/L, 1 μg/L, 10 μg/L, 20 μg/L, 30 μg/L, 40 μg/L, 50 μg/L, 100 μg/L ）, and set 48 h, 72 h two groups, then measure the apoptosis rate of bone marrow mesenchymal stem cells in each group by flow cytometry and analyze the data by statistical methods. Results The apoptosis rate of bone marrow mesenchymal stem cells could be reduced by Prostaglandin E1 at concentrations of 1, 10, 20, 30, 40, 50 and 100 μg/L, the lowest apoptosis rate was at 20 μg/L；Rat bone marrow mesenchymal stem cells have the lowest apoptosis rate at 48 hours. Conclusion The optimal concentration of prostaglandin E1 against apoptosis of rat bone marrow mesenchymal stem cells was 20 μg/L, and its anti?apoptotic effect peaked at 48 h in vitro.

Key words: prostaglandin E1, apoptosis, concentration, bone marrow mesenchymal stem cells