关键词: 三阴性乳腺癌, PI3K-AKT-c-Myc 信号通路, 增殖, 吗啡, 血管新生

Abstract: ［Abstract］ Objective To observe the influence of morphine on proliferation and vascular endothelial growth factor （VEGF） expression of triple-negative breast cancer cell （MDA-MB-231 breast cancer cell）, and to explore the effect of PI3K?AKT?c?Myc signal pathway on this process. Methods MDA?MB? 231 breast cancer cells were divided into negative control group（N group）, 1 μmol/L morphine group （1 μM group）, 10 μmol/L morphine group（10 μM group）and 100 μmol/L morphine group（100 μM group）randomly. The cells in N group were incubated with normal cultural medium without FPS , 1μM group were incubated with 1 μmol/L morphine, 10 μM group were incubated with 10 μmol/L morphine and 100 μM group were incubated with 100 μmol/L morphine. After the treatments, MTS was applied to detect the proliferation of MDA?MB?231 breast cancer cells, while the expression of vascular endothelial growth factor（VEGF）, c?Myc and the phosphorylation of AKT were detected by Western Blot. Results Compared with the N group, each concentration of morphine in our experiment could promote the prolifer? ation of MDA?MB?231 cells, increase the expression of VEGF, c?Myc and the phosphorylation of AKT. The 10 μM group increases most significant（P<0.05）. Conclusion Morphine in the concentration of 10 μmol/L could increase MDA?MB?231 breast cancer cell proliferation and VEGF expression, activation of the PI3K?AKT?c?Myc signal pathway may related to this process.

Key words: proliferation, morphine, triple-negative breast cancer, angiogenesis, PI3K-AKT-c-Myc signal pathway