关键词: TCGA 数据库, 乳腺癌, 组织芯片, 柠檬酸合成酶

Abstract: ［Abstract］ Objective To investigate the expression of citrate synthase in breast cancer tissues and non? cancerous tissues, and analyze the associations of its expression and clinical pathological features. Methods Western blot was used to detect the protein level of CS in breast cancer tissues and non-cancerous tissues. Immunohistochemistry was used to detect the expression of CS in breast cancer tissue microarrays. The associations of CS expression and clinical pathological features were analyzed. The relationship between CS expression and survival period of breast cancer patients in Cancer Genome Atlas （TCGA） database was analyzed. Results The expression level of CS protein was significantly up-regulated in breast cancer patients （P=0.028）. The expression of CS protein was correlated with histological grade（P=0.035）, clinical stage（P<0.001）, and lymph node metastasis（P<0.001）. The survival rate of breast cancer patients with high CS expression was significantly reduced （P=0.007）. Conclusion Our study showed that up?regulation of CS in breast cancer is related to the progression of breast cancer and its clinical prognosis. It is expected to be a new biomarker for breast cancer.

Key words: citrate synthase, tissue microarray, breast cancer, TCGA database