关键词: 临床意义, LINC00475, 长链非编码RNA, 肾癌

Abstract: Objective To investigate the expression and clinical significance of long non-coding RNA（lncRNA）LINC00475 in renal cell carcinoma（RCC）, and analyze the effect of LINC00475 on the proliferation and migration of 786?O cells. Methods The expression of LINC00475 in RCC and adjacent normal tissues was detected by qRT-PCR. The clinical significance and prognostic value of LINC00475 in RCC was analyzed using TCGA data.LINC00475 expression was down?regulated by trans-fecting siRNA into 786-O cells. Later, the proliferation and migration of 786-O cells were detected by MTS and transwell assays. Finally, the potential molecular mechanism of LINC00475 involved in RCC was analyzed by gene enrichment analysis （GSEA）. Results The expression of LINC00475 in RCC tissues was significantly higher than in normal tissues. The expression of LINC00475 was positively asso-ciated with tumor stage（P=0.010）and M stage（P=0.022）. Meanwhile, patients with higher LINC00475 expression had a shorter survival time than patients with lower LINC00475 expression. Moreover, knock-down LINC00475 could significantly inhibit the proliferation and migration of 786-O cells. Finally, GSEA revealed that the gene sets associated with cyclin D1, VEGFA, WNT signaling pathway were signif?icantly up-regulated in LINC00475 high expression group（NOM P<0.01, FDR Q<0.05）. Conclusion LINC00475 was overexpressed in RCC and knockdown LINC00475 could inhibited the proliferation and migration of 786?O cells. LINC00475 might be an important biomarker in RCC.

Key words: LncRNA, clinical significance, renal cell carcinoma, LINC00475