关键词: 磺达肝癸钠, 微循环, 高脂血症, 急性坏死性胰腺炎, 炎症因子, 大鼠

Abstract: ［Abstract］ Objective To investigate the effects of fondaparinux sodium on microcirculation and inflammatory factors in hyperlipidemic rats with acute necrotizing pancreatitis. Methods Thirty healthy male SD rats were taken as the research objects , and randomly divided into fondaparinux group（n=15） and hyperlipidemia group （n=15）. Both of the two groups were fed with high fat diet to establish hyperlipidemia rat model. In additional, fondaparinux group had 0.1 ml/100 g weight of 5% sodium taurocholate solution injected into pancreatic duct to make acute necrotizing pancreatitis model. 0 h, 6 h, 12 h and 18 h after model making, the animals in fondaparinux group had 0.2 ml/100 g fondaparinux sodium injection. After two groups model making , the rats were prohibited food taking and drinking , and given 2 ml/100 g NS rehydration injection. Before treatment and 24 h after treatment, both of the two groups had venous blood taken for detection of serum IFN?γ, TNF?α, IL?6 and IL?10. And before treatment and after treated for 6 h, 12 h and 24 h, pancreatic head and tail micro?blood flow in the two groups were examined and compared. Rats in two groups were killed of 24 h after model making. Laparotomy was performed for obaervation of intra?abdominal tissue and pancreas , and Removal of duodenal pancreatic tissue for pathological examination. Results Compared with the hyperlipidemia group, the levels of serum IFN?γ, TNF?α, IL?6 and IL?10 before treatment in fondaparinux group were increased（all P values <0.05）. Compared with before treatment, 24 h after treatment, levels of serum inflammatory factors in fondaparinux group was decreased （all P values <0.05）. The micro?blood flow volume of pancreas head and tail in fondaparinux group was significantly increased , when compared with the hyperlipidemia group and before fondaparinux treatment（all P values <0.05）. Pathological scores of edema, necrosis and inflammatory cell infiltration in the fondaparinux group were higher than those in the hyperlipidemia group （P<0.05）. ConclusionFondaparinux sodium can improve the microcirculation and inflammatory state of rats , so as to achieve the purpose of disease treatment.

Key words: fondaparinux sodium, hyperlipidemia, microcirculation；inflammatory factors, acute necrotizing pancreatitis, rats