关键词: 二胺氧化酶, 细菌内毒素, D-乳酸, 危重症

Abstract:

Objective To investigate the value of diamineoxidase, D-lactic and endotoxin in critical illness with gastrointestinal dysfunction. Methods  117 patients admitted to ICU were quantitatively detected serum diamineoxidase, D-lactic and endotoxin by using diamineoxidase/lactic/ endotoxin inspection Kit, and calculated the first 24 h Acute Physiology And Chronic Health Evaluation Ⅱ （APACHE Ⅱ ）. The gastrointestinal function of patients was evaluated firstly, and according to whether patients presented gastrointestinal dysfunction, 117 patients were assigned to two group:non-gastrointestinal dysfunction group and gastrointestinal dysfunction group. Results Plasma diamineoxidase level were markedly increased in gastrointestinal dysfunction group（16.46±22.60 U/L）as compare with non-gastrointestinal dysfunction group（6.33 ± 9.55 U/L, P=0.01）. Serum D-lactate levels were markedly increased in gastrointestinal dysfunction group（24.32 ± 16.49mg/L）as compare with non-gastrointestinal dysfunction group（11.66±12.36 mg/L, P<0.01）. Serum endotoxin levels were higher in gastrointestinal dysfunction group （11.43 ± 9.70U/L） than in non-gastrointestinal dysfunction group （9.34 ± 0.17 U/L, P=0.04） Diamineoxidase, D-lactic and endotoxin levels were positively correlated with APACHE II scores:r=0.427, P=0.01, r=0.53, P<0.001, and r=0.451, P<0.001, respectively. Conclusion Diamineoxidase, D-lactic and endotoxin levels were significant increasing in patients with severe gastrointestinal dysfunction and were positively correlated with APACHE and II scores. It suggests the serum diamineoxidase, D-lactic and endotoxin can be used to evaluate gastrointestinal dysfunction as well as the severity in critically ill patients.

Key words: diamineoxidase, D-lactate, endotoxin, ICU