关键词: 原发性肝细胞癌, 肿瘤转移, NEK9, 复发

Abstract:

 Objective To investigate the expression of NEK9 in hepatocellular carcinoma （HCC）tissues and its correlation with clinical pathological parameters. Methods Sixty cases of primary HCC tissues, paired normal tissues and their clinical data were collected in our hospital during 2010 to 2011. The mRNA level expression of NEK9 was determined by qRT-PCR. The protein expression of NEK9 was detected by immunohistochemical staining. Then the relationship of NEK9 expression and clinical pathological parameters were analyzed. Results Compared to the paired normal tissues, the expression of NEK9 was explored to be much higher in primary HCC tissues, both at mRNA level（25/30） （P<0.05）, and at protein level（65% and 21.7%）（P<0.05）. The expression of NEK9 was found to be correlated with tumor size, TNM stage metastasis and recurrence and tumor?free survival time（P<0.05）. Conclusion The over?expression of NEK9 is concerned with tumor size, TNM stage, metastasis, recurrence and tumor?free survival time, and maybe a potential marker for early diagnosis and prognosis of HCC.

Key words: NEK9, hepatocellular carcinoma, recurrence, metastasis