关键词: 骨髓间充质干细胞, 脱细胞海绵体支架, 组织工程化阴茎海绵体, CD34+ 单核细胞, 组织工程, 阴茎再造

Abstract:

Objective To investigate the feasibility of co?cultured mesenchymal stem cells（MSCs） and CD34 + mononuclear cells seeded in acellular matrix to construct a section of rabbit tissue?engineered cavernosum in vivo. Methods MSCs and CD34+ mononuclear cells （MNCs）were extracted from bone marrow and peripheral blood. After being identified，the two components of the cells were seeded in prepared acellular corporal matrix alongside with MSCs and CD34 + MNCs seeded in the same density respectively. The cell?laden matrix was transplanted into a rabbit model of penile interruption after culture for 4 days in vitro. Three months after implantation ，animals were killed and the engineered tissue was collected for histological examination （H&E staining and Masson′ s trichrome staining）. Results The engineered corporal tissue near tunica albuginea in MSCs and CD34+ MNCs cocultured group preserved the histological feather of intact corporal tissue. The other 3 groups，including MSCs group，CD34+ MNCs group and acellular matrix group，failed to preserve the corporal structure. Instead，they underwent fibrosis and formed scar tissue though samples from MSCs group showed more small vessels than from CD34+ MNCs group and acellular matrix group （P<0.05）. Conclusion Our work demonstrates the feasibility of co?implantation of MSCs and CD34+ MNCs in acellular matrix to construct corporal tissue resembling to native one. CD34+MNCs promote vascularization in vivo along with MSCs rather than alone.

Key words: CD34+Mononuclear Cells, acellular corporal matrix, tissue engineering, penile reconstruction, tissue?engineered corpus cavernosum, bone marrow?derived Mesenchymal stem cells