关键词: 白介素6, 肝再生磷酸酶-3, 结肠癌, 迁移

Abstract: 【Abstract】 Objective To investigate mechanism of cytokines of interleukin (IL)-6 in promoting the colorectal cancer cells proliferation and migration. Methods〓We used ELISA to detect the level of IL-6 in culture medium of TAMs after co-culture with control group:LoVo-PRL-3 (LoVo-P) andexperimental group:LoVo-Control(LoVo-C). Western blot was used to detect the proteins of p-AKT and p-STAT3. The migration and proliferation were measure by wound healing and MTS. Results〓Elisa assay displayed that the protein level of IL-6 of TAM/LoVo-P was higher than TAM/LoVo-C. Wound healing and MTS test both displayed that the migration and proliferation of LoVo-P were higher than LoVo-C when co-culture with TAM.Western blot reminded the proteins of p-AKT and p-STAT3 in LoVo-P were higher than LoVo-C after co-culture with TAMs. When we added different dosages of IL-6 (1、10、100 NG/ML) in culture medium of LoVo-P, the migration and proliferation were increased gradually, also the proteins of p-AKT and p-STAT3 gradient increased. Conclusion〓PRL-3 promotes colon cancer cells proliferation and migration depends on paracrine IL-6 signaling and up-regulate the proteins of p-AKT and p-STAT3.

Key words: IL-6, Colon cancer, PRL-3, Migration