染料木黄酮诱导前列腺癌DU-145细胞凋亡的研究

doi:10.3969/j.issn.1009-976X.2016.02.023

摘要/Abstract

摘要： 【摘要】〓目的〓探讨染料木黄酮诱导前列腺癌细胞系DU-145细胞凋亡的作用。方法〓在Caspase抑制剂Z-VAD存在或不存在的情况下,以染料木黄酮作用于前列腺癌DU-145细胞,采用MTT法检测细胞存活率、Annexin V-FITC/PI双染法流式细胞术检测细胞凋亡、PI染色法流式细胞术检测细胞周期、JC-1染色法流式细胞术测定细胞线粒体膜电位(△ψm),比色检测试剂盒测定caspase-8活性。结果〓染料木黄酮能明显降低前列腺癌DU-145细胞的存活率、诱导癌细胞凋亡、降低△ψm及提高caspase-8活性(全部P<0.05)。Z-VAD能够逆转Gen抗前列腺癌的效应。结论〓染料木黄酮使细胞阻滞于G0和G1,分别通过凋亡信号通路的外源性和内源性途径,诱导前列腺癌细胞凋亡。

关键词: 染料木黄酮, 前列腺癌, 凋亡

Abstract: 【Abstract】〓Objective〓To investigate the effects of genistein.(Gen).on the apoptosis of human prostate cancer DU-145 cells. Methods〓Human prostate cancer cell line DU-145 cells were treated with Gen with or without pan-caspase inhibitor Z-VAD(Z-VAD-FMK). Cell viability was examined by MTT assay..Apoptosis was determined using Annexin V-FITC/PI staining and flow cytometry..Mitochondrial membrane potential.(△ψm).was analyzed by JC-1 staining and flow cytometry. Caspase-8 activity was measured by colorimetric assay kits. Results〓Gen inhibited viability, induced apoptosis,.decreased △ψm and increased caspase-8 activity.(All P values were less than 0.05) in DU-145 cells. In addition, its effects could be reversed by Z-VAD. Conclusion〓Gen can induce apoptosis in human prostate cancer DU-145 cells by activation of both intrinsic and extrinsic apoptotic pathways.

Key words: Genistein, Prostate cancer, Apoptosis

中图分类号:

R737.25

萧聪勤胡建波周一南. 染料木黄酮诱导前列腺癌DU-145细胞凋亡的研究[J]. 岭南现代临床外科, 2016, 16(02): 215-218.

XIAO Congqin, HU Jianbo, ZHOU Yinan. Study of genistein on inducing apoptosis in prostate cancer DU-145 cells[J]. Lingnan Modern Clinics in Surgery, 2016, 16(02): 215-218.

参考文献

1. Engel-Nitz NM, Alemayehu B, Parry D, et al. Differences in treatment patterns among patients with castration-resistant prostate cancer treated by oncologists versus urologists in a US managed care population. Cancer Manag Res, 2011, 3: 233-245.
2. Sartor AO. Progression of metastatic castrate-resistant prostate cancer: impact of therapeutic intervention in the post-docetaxel space. J Hematol Oncol, 2011, 23(4): 18.
3. 许江兰, 范立. 前列腺癌的放射治疗. 肿瘤研究与临床, 2008, 20(12): 861-863.
4. 喻彬, 须霆, 邹青, 等. 激素抵抗性前列腺癌化疗15例报告. 现代肿瘤医学, 2010, 18(4): 748-749.
5. 曲妍妍, 张永忠. 染料木黄酮生物活性及抗病机理的研究进展. 食品工业技术, 2010, 31(8): 364-369.
6. Chen CD, Welsbie DS, Tran C, et al. Molecular determinants of resistance to anti-androgin therapy. Nature Medicine, 2004, 10(1): 33-39.
7. 周志耀, 戴玉田. 前列腺肿瘤. 见: 吴阶平主编, 吴阶平泌尿外科学. 济南: 山东科技出版社, 2004: 1059-1091.
8. Mansoor TA, Ramalho RM, Luo X, et al. Isoflavones as apoptosis inducers in human hapatoma HuH-7 cells. Phytocher Res, 2011, 25(12): 1819-1824.
9. Ma Y, Wang J, Liu L, et al. Genistein potentiates the effect of arsenic trioxide against human hepatocellular carcinoma: role of Akt and nuclear factor-κB. Cancer Lett, 2011, 301(1): 75-84.
10. 凌航, 陈裕庆, 高美钦, 等. Genistein联合5-FU对人结肠癌细胞株（SW480）增殖与凋亡的影响. 中华肿瘤防治杂志, 2010, 17(12): 912-915.
11. Raynal NJ, Momparler L, Charbonneau M, et al. Antileukemic activity od Genistein, a major isoflavone present in soy products. J Nat Prod, 2008, 71(1): 3-7.
12. Chipuk JE, Bouchier-Hayes L, Green DR. Mitochondrial outer membrane permeabilization during apoptosis: The innocent bystander scenario. Cell Death Differ, 2006, 13(8): 1396-1402.
13. Lucken-Ardjomande S, Martinou JC. Newcomers in the process of mitochondrial permeabilization. J Cell Sci, 2005, 118(pt 3): 473-483.
14. Nieminen AI, Partanen JI, Klefstrom J. c-Myc blazing a trail of death: coupling of the mitochondrial and death receptor apoptosis pathways by c-Myc. Cell Cycle, 2007, 6(20): 2464-2472.

[1]	张洋璠, 文金钡, 严颖哲, 黄晓燕, 谭梓聪, 杨浩杰, 纪风涛. 七氟烷通过影响神经元能量代谢和氧化应激水平促进神经元凋亡[J]. 岭南现代临床外科, 2022, 22(1): 30-33.
[2]	马晓武, 谭文亮, 杨磊, 谢智钦, 王庆斌, 陈亚进, 商昌珍. 环状RNA circKDM4B调控肝癌细胞增殖及侵袭迁移的实验研究[J]. 岭南现代临床外科, 2021, 21(06): 614-619.
[3]	朱传华, 谢琳, 刘安民. PDIA4基因沉默对胶质瘤细胞凋亡的影响[J]. 岭南现代临床外科, 2021, 21(02): 177-181.
[4]	陈秀凤, 卜庆丰, 张杰. 基于多参数磁共振成像的PI-RADS第二版评分联合f/t PSA及PSAD在前列腺癌早期筛查中的预测价值[J]. 岭南现代临床外科, 2021, 21(02): 213-216.
[5]	林杰, 刘清波, 何威, 马靖, 陈韵壕, 王卫东. 8-Nitroguanine在肝吸虫相关性肝内胆管癌中的表达以及意义[J]. 岭南现代临床外科, 2021, 21(01): 59-62.
[6]	袁红, 胡小芳, 陈业辉, 李淑霞. 基于微视频健康教育在前列腺癌根治术后留置尿管患者护理中的初步观察[J]. 岭南现代临床外科, 2020, 20(05): 677-680.
[7]	陈美贤, 杨雪莹, 廖朝霞, 叶西就, 陆福鼎. “深肌松”在肥胖患者行机器人辅助腹腔镜下根治性前列腺癌切除术中的应用观察[J]. 岭南现代临床外科, 2020, 20(04): 468-471.
[8]	袁小旭, 邱剑光, 朱雨沫, 林雪花, 庞国福, 邓建, 谢群. 经会阴前列腺穿刺与经直肠前列腺穿刺活检在前列腺癌诊断的初探[J]. 岭南现代临床外科, 2020, 20(04): 484-487.
[9]	叶惠荣，吴丽华，张玉娟，高学忠，王西跃，曹茵. 灵菌红素通过促进Bim调控人乳腺癌细胞凋亡[J]. 岭南现代临床外科, 2020, 20(01): 38-43.
[10]	胡丽娟，戴奇山，胡建波*. 89锶、唑来膦酸及99锝-亚甲基二膦酸盐治疗老年前列腺癌骨转移的临床观察[J]. 岭南现代临床外科, 2019, 19(04): 437-446.
[11]	胡丽娟，戴奇山，胡建波*. 阿比特龙联合泼尼松治疗老年转移性去势抵抗前列腺癌的临床观察[J]. 岭南现代临床外科, 2019, 19(03): 333-336.
[12]	尤洪科1，文博1，尹永华1. 去泛素化酶3在前列腺癌中的表达及其对前列腺癌细胞生物学特性的影响[J]. 岭南现代临床外科, 2019, 19(03): 337-340.
[13]	叶秀钦，丁志敏，董发进，魏章洪，刘慧玉，吴淮宇，徐金锋*. 经直肠实时弹性成像引导靶向穿刺在前列腺病变的应用价值研究[J]. 岭南现代临床外科, 2019, 19(02): 195-198.
[14]	刘丛勇，张露，苏峰，邓保平，曾宽，杨艳旗*. 前列腺素E1 抗大鼠骨髓间充质干细胞凋亡的最佳浓度探讨[J]. 岭南现代临床外科, 2018, 18(05): 504-508.
[15]	林春浩，赖义明，彭圣萌，黄海，郭正辉. Polo样激酶3在前列腺癌中的表达及临床意义[J]. 岭南现代临床外科, 2018, 18(02): 133-136.