关键词: 基因转染技术, 局部治疗, 肝转移癌, 兔

Abstract: 【Abstract】〓Objective〓This work aims to investigate its effect on angiogenesis of hepatic colorectal metastasis,and to elucidate its molecular mechanism. Methods〓Recombinant expressing plasmids harboring wild type p53 and Rb were cotransferred or transferred separately to the mice hepatic VX2 tumor by the emulsion of Pll-nHAP nanoplex and lipodiol through the hepatic artery in a tumor target manner. Subsequent co-expressions of p53 and Rb protein within the treated tumors were investigated by western blotting and in situ analysis of confocal Laser scanning microscope. The therapeutic effect on angiogenesis was evaluated by the investigation of the microvessel density of tumor tissue. Eventually, the real-time RT-PCR and ECL western bolt were applied to investigate the expressive changes of important genes related to the above. Results〓(p53+Rb) LNT showed significantly better anti-angiogenesis effect and lower expression of related CD31, CD34, VEGF genes than the control group, although no significant difference was observed compared to p53 or Rb LNT for some genes. Conclusion〓Rb work synergistically with p53 in combined therapy mediated by Pll-nHAP nanoplex to augment the antitumoral effect through the down-regulated expression of important genes related to angiogenesis. Our study revealed that LNT with p53 and Rb is a potentially effective antitumor therapy for HCC.

Key words: Nanoparticles, Hepatic Metastasis, Gene Transfer Techniques, Rabbits