关键词: 脓毒血症, HMGB1, 自噬调控, 肝细胞损伤, NALP3炎性体

Abstract: 【Abstract】 Objective To study the mechanism of high migration rate group protein B1(HMGB1) migration mediated autophagy protects the liver cells injury in sepsis. Methods〓Seventy-two male Lewis rats were randomly divided into endotoxin group (n=18), autophagy induction group (n=18), autophagy inhibition group (n=18) and control group (n=18). The rats in endotoxin group was given intraperitoneal injection of endotoxin (LPS, 25 mg/kg) to induce endotoxemia. Rats in autophagy induction group and inhibition group received intraperitoneal administration of rapamycin or ward penicillin pre stimulation respectively, and followed establishment of endotoxemia one hour later. Rats in blank group were treated with intraperitoneal injection of Ringer's solution(1 ml). The changes of HMGB1 and autophagy in liver tissue, liver function and cytokines were observed on the time points of 6 h, 24 h, 48 h after operation. Results〓In the endotoxin group, the autophagy of hepatocytes, the HMGB-1 in hepatocytes cytoplasm,the liver enzymes in serum and IL-1β levels increased significantly (P<0.01). However, the level of HMGB1, liver enzymes and IL-1β in the rats of autophagy induction group significantly decreased (P<0.01) compared with the endotoxin group. Conclusion〓In the sepsis, HMGB1 migration mediated autophagy regulates in IL-1β to reduce liver cell injury and promote the recovery of liver function.

Key words: Sepsis, HMGB1, Autophagy regulation, Liver damage, NALP3 inflammasome