关键词: 乳腺癌, 软琼脂模型, 壳聚糖支架模型, 化疗药物敏感性

Abstract: 【Abstract】〓Objective〓To compare the difference of the epirubicin sensitivity of the breast cancer cells between three dimensional soft agar and chitosan scaffold models. Methods〓The growth curve of breast cancer cells separately cultured in three dimensional soft agar and chitosan scaffold models were recorded by cell counting and colony counting assay, then the epirubicin sensitivity of breast cancer cells were measured by MTT assay and colony counting assay, and the IC50s of these cells were calculated by IC50 calculating software and the difference between the IC50s was analyzed by t test. Results〓Both in three dimensional soft agar and chitosan scaffold models, the breast cancer MCF-7 and 231 cells could reach a good growth state; the IC50s of the breast cancer cells between three dimensional soft agar and chitosan scaffold models were not significantly different (P>0.05). Conclusion〓The chemosensitivity of breast cancer cells are not different in three dimensional soft agar and chitosan scaffold models, which indicate that the three dimensional growing environment doesn’t significantaly impact the the chemosensitivity of breast cancer cells.

Key words: Breast cancer, Soft agar model, Chitosan scaffold model, Chemosensitivity